B. Vázquez, C. Fente, C. Franco, A. Cepeda, G. Mahuzier, P. Prognon
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引用次数: 19
摘要
柱后荧光法测定黄曲霉毒素Q1、P1和B1，色谱柱为2.0 mm。柱后试剂为10-2M的庚-二o -甲基-β-环糊精水溶液。AFQ1和AFB1的荧光增强分别为37倍和27倍，而AFP1信号仅增加约2倍。该方法可同时测定人体尿液中的黄曲霉毒素Q1、P1和B1。检出限(S/N = 3)为:AFQ1为0.7 ng ml-1, AFP1为0.5 ng ml-1, AFB1为0.3 ng ml-1。
Preliminary study on fluorimetric detection of aflatoxins Q1, P1 and B1 using heptakis-di-O-methyl-β-cyclodextrin as post-column HPLC reagent
Post-column fluorimetric detection for the determination of aflatoxins Q1, P1 and B1 was carried out by using HPLC with a 2.0 mm id column. The post-column reagent consisted of a 10–2M aqueous solution of heptakis-di-O-methyl-β-cyclodextrin. The fluorescence enhancement achieved was 37- and 27-fold for AFQ1 and AFB1, respectively, whereas the AFP1 signal was increased just about 2-fold. With the proposed method, aflatoxins Q1, P1 and B1 can be simultaneously determined in human urine. The detection limits (S/N = 3) were as follows: 0.7 ng ml–1 for AFQ1, 0.5 ng ml–1 for AFP1 and 0.3 ng ml–1 for AFB1.
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