尼泊尔rupendehi地区菖蒲根茎挥发油化学成分及抑菌活性研究

S. Joshi, Sundar Bashyal
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引用次数: 9

摘要

采用Clevenger-type加氢蒸馏装置从野生菖蒲根茎风干粉末中分离得到精油,并采用气相色谱-质谱联用技术对其化学成分进行了分析。共鉴定和量化了14种化学成分,占总油成分的100%。主要化学成分为β-asarone(68.96%)、4-羟基-4-甲基-2-戊酮(8.73%)、shyobunone(8.65%)、对甲氧基苯基丁酮(4.16%),次要化学成分为δ-cadine(0.84%)、isoemicine(2.68%)、murolene(1.92%)和顺辛烯(1.44%)。采用纸盘法对野生菖蒲根状茎挥发油对1株金黄色葡萄球菌革兰氏阳性菌和3株大肠杆菌、肺炎克雷伯菌和寻常变形杆菌进行抑菌试验。从菖蒲根茎干粉中分离得到的精油对被试细菌具有良好的抑菌潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
STUDY ON THE CHEMICAL CONSTITUENTS AND ANTIBACTERIAL ACTIVITY OF ESSENTIAL OIL OF ACORUS CALAMUS L. RHIZOMES OF RUPENDEHI DISTRICT (NEPAL)
The essential oil from the air dried powder of wild Acorus calamus L. rhizomes was isolated using Clevenger-type hydrodistillation apparatus and its chemical constituents were analyzed by gas chromatography-mass spectrometric (GC-MS) analysis. A total numbers of fourteen chemical constituents were identified and quantified occupying 100 % of total oil composition. The major chemical constituents are reported to be β-asarone (68.96 %), 4-hydroxy-4-methyl-2-pentanone (8.73 %) shyobunone (8.65 %), p-methoxyphenyl butanone (4.16 %) and the minor chemical constituents are δ-cadinene (0.84 %), isoelemicine (2.68 %), muurolene (1.92 %) and cis-ocimene (1.44 %). The antibacterial activity of the essential oil isolated from the wild Acorus calamus L. rhizomes was carried out using paper disc method against one Gram positive bacteria of Staphylococcus aureus and three Gram negative bacteria of Escherichia coli, Klebsiella pneumoniae and Proteus vulgaris. The isolated essential oil from the dry powder of Acorus calamus L. rhizome showed a good potentiality of the antibacterial effect against tested bacteria.
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