鼠疫疫苗接种人鼠疫耶尔森氏菌抗原对体外t辅助细胞活化的影响

V. A. Kozhevnikov, A. L. Kravtsov, O. M. Kudryavtseva, T. Kashtanova, S. Bugorkova
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摘要

的相关性。寻找用于评估鼠疫活疫苗免疫功效的信息性标记物仍然是一项紧迫的科学任务。该研究的目的是在体外试验中比较评估接种鼠疫疫苗的人体内CD69和HLA-DR标记的辅助性t细胞激活水平,使用超声波分解的鼠疫菌细胞,在28°C温度下生长作为特异性刺激物。材料与方法。对接种鼠疫疫苗的45人的血液样本进行了细胞荧光分析。结果表明,两种细胞标记物的细胞学分析结果与免疫供体接种时免疫调节指数(IRI)的相关性。结果发现,对于新接种的个体,早期激活标记对辅助t细胞的应答更为强烈和持久,而对于再次接种免疫调节指数的个体,在接种时间大于1.5时,晚期激活标记对辅助t细胞的应答更为强烈。因此,在鉴定特定抗原刺激时淋巴细胞活化标记物的基础上,对鼠疫疫苗免疫效果进行定量评价的可能性得到了证实。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Activation of T-helper in vitro under the Influence of Yersinia pestis Antigens in People Vaccinated against the Plague
Relevance. The search for informative markers for assessing the immunological efficacy of a live plague vaccine remains an urgent scientific task. The goal of the studyAims was to make a comparative assessment of the level helper T-cells activation by CD69 and HLA-DR markers, in people, vaccinated against plague, in an in vitro test, using the disintegrated by ultrasonic Y. pestis cells, grown at temperature 28 °C as a specific stimulator.Materials & Methods. A cytofluorimetric analysis of blood samples of 45 individuals vaccinated against the plague was carried out. The dependence of the result of cytological analysis of the two studied cell markers on the immunoregulatory index (IRI) of the vaccinated donor at the time of vaccination was revealed.Results. It was found that for newly vaccinated individuals, the T-helpers were more intensive and prolonged by the early activation marker, while for those revaccinated with the immunoregulatory index, at the time of vaccination more than 1.5, the more intense cellular response was by the late activation marker.Conclusions. Thus, the possibility of quantitative evaluation of the immunological efficacy of vaccination against plague, based on the identification of lymphocyte activation markers when stimulated with a specific antigen, is confirmed.
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