顽固性种子物种山茶种子发育过程中的二维凝胶电泳蛋白谱分析

L. L. C. Dias, T. S. Balbuena, V. Silveira, C. Santa‑Catarina, A. Schevchenko, E. Floh
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引用次数: 14

摘要

本研究的目的是利用双向凝胶电泳技术,研究一种抗性植物O. catharinensis种子发育过程中蛋白质谱的变化。用硫脲/尿素缓冲液提取蛋白质,然后用10% TCA沉淀。种子发育过程的比较分析表明,在种子发育的每个阶段都有大量的特异性蛋白被检测到。子叶期是胚胎发生和成熟相关代谢开始的过渡阶段,具有最多的阶段特异性斑点。蛋白质鉴定,通过MS/MS分析,鉴定出主要与氧化代谢和储存合成有关的蛋白质。这些发现有助于更好地理解顽固性种子种子发育过程中的蛋白质代谢,此外还提供了有关可用于定义和改进体细胞胚胎发生方案的已建立标记的信息,以及监测该物种体细胞胚胎的发育。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Two-dimensional gel electrophoretic protein profile analysis during seed development of Ocotea catharinensis: a recalcitrant seed species
The aim of the present work was to characterize changes in the protein profile throughout seed development in O. catharinensis, a recalcitrant species, by two-dimensional gel electrophoresis. Protein extraction was undertaken by using a thiourea/urea buffer, followed by a precipitation step with 10% TCA. Comparative analysis during seed development showed that a large number of proteins were exclusively detected in each developmental stage. The cotyledonary stage, which represents the transition phase between embryogenesis and the beginning of metabolism related to maturation, presents the highest number of stage-specific spots. Protein identification, through MS/MS analysis, resulted in the identification of proteins mainly related to oxidative metabolism and storage synthesis. These findings contribute to a better understanding of protein metabolism during seed development in recalcitrant seeds, besides providing information on established markers that could be useful in defining and improving somatic embryogenesis protocols, besides monitoring the development of somatic embryos in this species.
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