广西杂交石斑鱼(Epinephelus fuscoguttatus♀× Epinephelus lanceolatus♂)神经坏死病毒的分离

IF 0.6 4区农林科学 Q4 FISHERIES

Fish Pathology Pub Date : 2019-03-15 DOI:10.3147/JSFP.54.16

Qing Yu, Mingzhu Liu, Shina Wei, Hehe Xiao, Siting Wu, Xianling Qin, Deqiang Shi, Siqiao Li, Taixia Wang, Pengfei Li

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引用次数: 14

摘要

——2018年8月，中国广西养殖石斑鱼出现疑似病毒性神经坏死疫情。病理组织学显示，患病杂交石斑鱼幼鱼(斑石斑鱼♀× lanceolatus Epinephelus♂)脑组织有严重空泡化。用石斑鱼细胞系EAGB从脑和眼混合样本中分离出一种神经坏死病毒(NNV)。在EAGB细胞中出现了多个细胞质空泡细胞的细胞病变效应。该分离物(GNNV-Gx)外壳蛋白基因序列表明，该分离物属于RGNNV基因型，与GenBank中登记的一种虎石斑鱼NNV外壳蛋白基因亲缘关系最为密切。在pH为3和pH为10时，菌株的传染性显著降低，对60°C敏感。

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Isolation of Nervous Necrosis Virus from Hybrid Grouper (Epinephelus fuscoguttatus♀ × Epinephelus lanceolatus♂) Cultured in Guangxi, China

― Outbreaks of suspected viral nervous necrosis emerged in cultured groupers in Guangxi, China in August 2018. Histopathology showed severe vacuolation in the brain tissue of the diseased juvenile hybrid grouper ( Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂ ). A nervous necrosis virus (NNV) was isolated from pooled sample of the brain and eyes using a grouper cell line EAGB. Cytopathic effect with multiple cytoplasmic vacuolation cells developed in EAGB cells. The coat protein gene sequence of the isolate (GNNV-Gx) showed that it belonged to RGNNV genotype and was most closely related to that of a tiger grouper NNV registered in GenBank. The infectivity of the isolate decreased remarkably at pH 3 and pH 10 and was susceptible to 60 ° C.

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