大黄对HepG2细胞株遗传毒性的体外评价

Mahmoud Abudayyak
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引用次数: 3

摘要

大黄是一种多年生草本植物,属于蓼科,多生长在黎凡特和土耳其高海拔地区的岩石和砾石斜坡上。大黄被作为食物食用,并广泛用于民间医学,用于治疗恶心、便秘和不同的疾病,包括糖尿病和高血压。遗憾的是,对大黄毒理的研究还不够。本研究采用MTT法和NRU法对大黄水、甲醇和氯仿提取物对人肝癌(HepG2)细胞株进行细胞毒性评价。采用彗星法研究植物提取物的遗传毒性。我们的研究结果表明,在5-50 mg/mL浓度下,所有提取物都以浓度依赖的方式导致细胞死亡。MTT法IC50值为14.29 ~ 31.94 mg/mL, NRU法为21.15 ~ 27.66 mg/mL。最高浓度(25 mg/mL)的甲醇提取物对DNA造成显著损伤(8.7倍)。综上所述,与民间医药中使用的许多植物类似,大黄的风险仍然未知。不加控制地使用这种植物会对病人有害。我们的研究结果提示大黄可能具有细胞毒性和遗传毒性,这些结果应该引起人们对大黄和其他民间草药安全性的关注。本文引自:Abudayyak M(2019)。大黄对HepG2细胞株遗传毒性的体外评价。IstanbulJPharm 10.26650/ istanbuljpharma .2019.19021。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
In vitro evaluation of Rheum ribes induced genotoxicity in HepG2 cell lines
Rheum ribes is a perennial herbaceous plant belonging to the Polygonaceae family that grows more in rocky and gravelly slopes in high altitude areas of Levant and Turkey. Rheum ribes is consumed as food and widely used in folk medicine against nausea, constipation and for different diseases including diabetesand hypertension. Unfortunately, the researches on Rheum ribes toxicity are insufficient. In our study, human hepatocellular carcinoma (HepG2) cell line was used in cytotoxicity evaluation of Rheum ribes water, methanol and chloroform extracts by MTT and NRU test. Comet assay was used to investigate the genotoxicity potentials of the plant extracts. Our results show that all extracts cause cell death in a concentration dependent manner at 5-50 mg/mL concentrations. The IC50 values are 14.29- 31.94 mg/mL by MTT and 21.15 - 27.66 mg/mL by NRU assay. The highest concentration (25 mg/mL) of methanol extract causes a significant DNA damage (8.7-folds). In conclusion, similar to a lot of plants used in the folk medicine the risk of Rheum ribes is still unknown. The uncontrolled use of this plant could harm to the patients. Our results indicate possible cyto- and gentoxicity effects of Rheum ribes, these results should rise the concerns about the safety of Rheum ribes and other folk herbs. Cite this article as : Abudayyak M (2019). In vitro evaluation of Rheum ribes induced genotoxicity in HepG2 cell lines. Istanbul J Pharm 10.26650/IstanbulJPharm.2019.19021.
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