耐药菌株具有不同的生物膜形成能力和不同生物膜相关基因的表达

African Journal of Traditional, Complementary and Alternative Medicines Pub Date : 2017-10-01 DOI:10.21010/AJTCAM.V14I5.8

Y. Tong, Lili Dou, Ye Sun, Dong-kai Zhao

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引用次数: 2

摘要

背景:引起非复杂性尿路感染(UTIs)的大肠杆菌菌株的耐药机制和模式差异很大。尿路致病性大肠杆菌(UPEC)的出现已成为一个严重的健康问题，但其潜在机制尚不清楚。方法:为探讨引起非复杂性uti的大肠杆菌的潜在耐药机制，对临床分离的3株UPEC耐药菌株(1351、4996、5028)进行了生物膜形成能力及pga ABCD和luxS基因的表达分析。结果:我们发现pgaA和pgaB的表达在1351和5028之间有很大差异，而luxS在4996中的功能缺失对生物膜的形成有影响。结论:1。菌株的抗药能力与其形成生物膜的能力有关。2. 耐药菌株的生物膜形成能力对葡萄糖的存在表现出不同的反应。3.luxS编码的AI-2对该菌株的生物膜形成不是必需的，但可能有助于更多生物膜的形成。

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HERB-RESISTANT UPEC STRAINS HAVE DIFFERENT BIOFILM FORMATION ABILITIES AND DIFFERENT EXPRESSION OF CERTAIN BIOFILM RELATED GENES

Background: The mechanisms and patterns of drug resistances of E. coli strains that cause uncomplicated urinary tract infections (UTIs) vary considerably. The emerging herbresistance of uropathogenic Escherichia coli (UPEC) has been a serious health problem, yet with unknown underlying mechanisms. Methods: To explore the potential herb-resistance mechanisms of E. coli strains that cause uncomplicated UTIs, three clinically isolated herb-resistant UPEC strains (1351, 4996, 5028) were analyzed for their abilities to form biofilms and the expressions of the pga ABCD and luxS genes. Results: We found that the expression of pgaA and pgaB are very different between 1351 and 5028, and the loss-offunction of luxS in 4996 has impact on biofilm formation. Conclusions: 1. Herb-resistance of the strains is related to their abilities of biofilm formation. 2. Biofilm formation capabilities of herb-resistant strains show different responses to the presence of glucose. 3. luxS encoded AI-2 is not essential for biofilm formation in this strain but may help with more biofilm formation.

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来源期刊

African Journal of Traditional, Complementary and Alternative Medicines 医学-全科医学与补充医学

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The “African Journal of Traditional, Complementary and Alternative Medicines (AJTCAM)” is a peer-reviewed, multidisciplinary, international, scientific Open Access Journal that provides publication of articles on phytomedicines, ethnomedicines and veterinary ethnomedicines. The journal is published by a Non-Governmental Organization (NGO) known as “African Traditional Herbal Medicine Supporters Initiative (ATHMSI)”. The Journal welcomes submission of manuscripts that meet the general criteria of significance and scientific excellence. Papers will be published approximately two-to-three months after acceptance