抗mirna 144和抗mirna 150对PBMC中α珠蛋白链表达的影响

Rini Riyanti
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引用次数: 0

摘要

过量的未结合α珠蛋白链是导致重度β地中海贫血临床症状的基本病理生理机制。近年来有许多替代疗法通过增加γ珠蛋白链来减少未结合α珠蛋白链的影响。通过减少α珠蛋白链的替代疗法并没有引起太多的注意。α珠蛋白的表达涉及转录因子和mirna的复杂调控。它涉及GATA-1、KLFD和MYB转录因子。它还涉及到miRNA-144和miRNA-150。miRNA-144和miRNA-150在严重β地中海贫血患者中降低α珠蛋白链表达的作用是一种替代疗法。miRNA-144和miRNA- 150的活性需要通过anti-miRNA 144和anti-miRNA 150来确定。分析抗mirna 144和抗mirna 150对α珠蛋白链表达的影响。本研究是利用PBMC对1例重度β地中海贫血患者进行的实验性研究。PBMC分为未转染、anti-miRNA 144转染、anti-miRNA 150转染、anti-miRNA 144和anti-miRNA 150转染四组。qPCR检测miRNA-144和miRNA-150的表达情况。Western blot检测α珠蛋白链的表达。转染抗miRNA-144的重度β地中海贫血患者PBMC中miRNA-144的表达明显低于未转染的患者。结果与miRNA-144的表达一致,miRNA-144的表达比对照组低0.17倍。转染抗mirna 150后,重度β地中海贫血患者PBMC中miRNA-150的表达明显低于未转染的患者。与miRNA-150的表达结果一致,miRNA-150的表达比对照组低0.30倍。转染抗mirna 150的PBMC地中海贫血患者的α珠蛋白链表达明显低于未转染的患者。转染抗mirna 150和抗mirna 144的PBMC地中海贫血患者的α珠蛋白链表达明显低于未转染的患者。10-25 KDa波段的面积减小证明了这一点。本研究表明,抗mirna 150或抗mirna 144和抗mirna 150能够降低重度β地中海贫血患者PBMC中α珠蛋白链的表达。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
EFFECT OF Anti-miRNA 144 AND Anti-miRNA 150 ON THE EXPRESSION OF α GLOBIN CHAINS IN PBMC
The excess of unbound α globin chains are the basic pathophysiology of the cause of clinical symptoms in major β thalassemia. Recently there are many alternative therapies by increasing γ globin chains to reduced the effects of unbound α globin chains. Alternative therapies by decreasing α globin chains have not been much noticed. α globin expression involves complex regulatory involving transcription factors and miRNAs. It involves GATA-1, KLFD and MYB transcription factors . It also involves miRNA-144 and miRNA-150. The role of miRNA-144 and miRNA-150 to reduce the α globin chains expression in major β thalassemia patients is an alternative therapy. miRNA-144 and miRNA150 activity need to be known by using anti-miRNA 144 and anti- miRNA 150. Analyzed the effect of anti-miRNA 144 and anti-miRNA 150 on the expression of α globin chains. This study was an experimental study using PBMC of a major β thalassemia patients. PBMC divided into four groups that were not transfected, transfected by anti-miRNA 144, transfected by anti-miRNA 150 and transfected by anti-miRNA 144 and anti-miRNA 150. qPCR examinations to find out expression of miRNA-144 and miRNA-150. Western blot examination to find out the expression of α globin chains. There was significantly lower miRNA-144 expression in PBMC of major β thalassemia patients who had been transfected by anti-miRNA 144 than those not transfected. In line with the result of miRNA-144 expression which was 0.17 times lower than the control group. There was significantly lower miRNA-150 expression in PBMC of major β thalassemia patients who had been transfected by anti-miRNA 150 than those not transfected. In line with the result of miRNA-150 expression which was 0.30 times lower than the control group. There was significantly lower α globin chains expression in PBMC thalassemia patients who had been transfected by anti-miRNA 150 than those not transfected. There was significantly lower α globin chains expression in PBMC thalassemia patients who had been transfected by anti-miRNA 150 and anti-miRNA 144 than those not transfected. This is evidenced by the decreased in the area 10-25 KDa band. Based on this study, the administration of anti-miRNA 150 or anti-miRNA 144 and anti-miRNA 150 is capable of decreasing the expression α globin chains in PBMC of major β thalassemia patients.
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