基于分子标记的印尼优质榴莲品种多样性研究

Anggi Angeliena, A. Ma’ruf, Husni Ahmad Sidiq, Y. Anggraito, N. A. Habibah, F. Huyop, A. Retnoningsih
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引用次数: 3

摘要

印尼榴莲品种繁多。已有一百多个品种被认定为优质榴莲。优质榴莲的主要特点表现在滋味、质地和颜色上的优越。榴莲的果实鉴定和特性鉴定需要较长的时间,因为榴莲在种植后至少要4年才能结出果实。本研究旨在利用ISSR分子标记揭示印尼优质榴莲的多样性。从印度尼西亚5个地点(廖内群岛省昆都尔地区、西爪哇省茂物县、东爪哇省Banyuwangi县、龙目岛和中爪哇省Brongkol ambarawasub区)采集了55个优质榴梿品种的DNA样本。采用11种ISSR标记对样品进行分析。对优良榴莲品种DNA进行扩增,产生83个多态等位基因。在PKBT 9标记的13个等位基因分析中获得最高等位基因,在ISSR 10标记的5个等位基因分析中获得最低等位基因。55份印尼优质榴莲的ISSR标记多样性非常高,多态性率达93.25%。结果表明,55个榴莲品种间存在差异。相似度分析表明,没有一个品种具有100%的相似值。印尼榴莲品种繁多。已有一百多个品种被认定为优质榴莲。优质榴莲的主要特点表现在滋味、质地和颜色上的优越。榴莲的果实鉴定和特性鉴定需要较长的时间,因为榴莲在种植后至少要4年才能结出果实。本研究旨在利用ISSR分子标记揭示印尼优质榴莲的多样性。从印度尼西亚5个地点(廖内群岛省昆都尔地区、西爪哇省茂物县、东爪哇省Banyuwangi县、龙目岛和中爪哇省Brongkol ambarawasub区)采集了55个优质榴梿品种的DNA样本。采用11种ISSR标记对样品进行分析。对优良榴莲品种DNA进行扩增,产生83个多态等位基因。最高等位基因来自PKBT 9标记,多达13个等位基因,最低等位基因来自PKBT 9标记。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The diversity of superior Indonesian durians based on molecular markers
Indonesia has high diversity of Durian. More than a hundred cultivars have been recognized as superior durians. The main characteristics of superior durian are seen from superiority of taste, texture and color of arillus. Fruit identification and characterization of durian requires a relatively long periods because fruit producing minimum 4 years after planting. Aim of this study was to reveal the diversity of superior Indonesian durians using ISSR molecular markers. DNA samples were collected from 55 superior durian cultivars from five sites in Indonesia (Kundur region in Riau Islands province, Bogor regency in West Java province, Banyuwangi regency in East Java province, Lombok island, and Brongkol Ambarawasub-district in Central Javaprovince). Samples were analyzed using 11 ISSR markers. Amplification of superior durian cultivar DNA produced 83 polymorphic alleles. The highest allele was obtained from the analysis of PKBT 9 markers as many as 13 alleles and the lowest one was taken from the analysis of ISSR 10 markers in 5 alleles. The diversity of 55 superior Indonesian durians using ISSR marker was very high with a polymorphic percentage reached 93.25%. The results of this study indicated that all 55 durian cultivars are different. Similarity analysis showed that none of cultivars had a 100% similarity value.Indonesia has high diversity of Durian. More than a hundred cultivars have been recognized as superior durians. The main characteristics of superior durian are seen from superiority of taste, texture and color of arillus. Fruit identification and characterization of durian requires a relatively long periods because fruit producing minimum 4 years after planting. Aim of this study was to reveal the diversity of superior Indonesian durians using ISSR molecular markers. DNA samples were collected from 55 superior durian cultivars from five sites in Indonesia (Kundur region in Riau Islands province, Bogor regency in West Java province, Banyuwangi regency in East Java province, Lombok island, and Brongkol Ambarawasub-district in Central Javaprovince). Samples were analyzed using 11 ISSR markers. Amplification of superior durian cultivar DNA produced 83 polymorphic alleles. The highest allele was obtained from the analysis of PKBT 9 markers as many as 13 alleles and the lowest one was taken from the analysis of...
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