槲皮素对果蝇紫外线损伤的改善作用

S. Majumder, Mohna Bandyopadhyay, Sandip Pal, D. Mukhopadhyay
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引用次数: 1

摘要

槲皮素是一种植物类黄酮,存在于各种水果、茶叶、蔬菜等叶子中,因其抗氧化、抗癌、抗炎和抗神经变性作用而被广泛研究。紫外线辐射对人体是有害的,因为它可能导致皮肤癌等几种并发症。果蝇(Drosophila sp.)长期以来一直被用作遗传相关研究的节肢动物模型。本研究以黑胃果蝇为研究对象,评价槲皮素对UV-C辐射损伤的保护作用。槲皮素(10µM)预处理可恢复因紫外线照射而缩短的寿命,增加羽化率,且槲皮素的剂量低于先前报道的其他类黄酮的剂量。中等剂量紫外线照射后的果蝇表现出明显的腹部色素不全、翅膀卷曲或翅膀展开等异常特征,而槲皮素预处理后的果蝇没有出现这些异常特征或突变表型。成虫的闭合尺寸、蛹的大小和蛹的迁徙距离也有相当大的变化。凝胶电泳研究表明槲皮素对黑腹巨鳄唾液腺DNA具有保护作用。因此,槲皮素可能是一种有效的抗紫外线辐射损伤的保护剂。DOI: http://dx.doi.org/10.5281/zenodo.3588543
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Ameliorating effect of quercetin against UV radiation-induced damage in Drosophila melanogaster
Quercetin is a plant flavonoid found in various fruits, leaves such as tea, vegetables and has been extensively studied due to its antioxidative, anticancer, anti-inflammatory and anti-neurodegenarative effects. UV radiation is harmful for human being as it may cause several complications such as skin cancer. Fruit fly (Drosophila sp.) has long been used as an arthropod model for genetics related studies. In the present study, the protective effect of quercetin is evaluated against UV-C radiation induced damage using Drosophila melanogaster. Pre-treatment with quercetin (10 µM) recovered the shortened lifespan caused by UV radiation and has also increased eclosion rate and the dose of quercetin is lower than the previously reported doses of other flavonoids. Flies subjected to moderate dose of UV radiation showed distinct abnormal characters such as incomplete abdominal pigmentation, curly wings or outstretched wings, whereas quercetin pretreatment showed no such abnormal characters or mutant phenotypes.  There is a considerable amount of change in the eclosed adult fly size, pupal size and pupal migration distance as well. Gel electrophoresis study of salivary gland DNA of D. melanogaster demonstrates the efficacy of quercetin in conferring protection to DNA against UV radiation-induced damage. Therefore, it can be concluded that quercetin may act as an effective protective agent against UV radiation-induced damage. DOI: http://dx.doi.org/10.5281/zenodo.3588543
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