{"title":"阴离子对atp诱导的NG108-15细胞[Ca2+]增加的影响。","authors":"T. Watano, I. Matsuoka, K. Ogawa, J. Kimura","doi":"10.1254/JJP.89.302","DOIUrl":null,"url":null,"abstract":"We investigated the effects of anions on different P2 receptors by measuring ATP-induced increase in intracellular Ca2+ concentration ([Ca2+]i) in fura-2-loaded NG108-15 and PC12 cells. In NG108-15 cells, ATP at 100 microM and 1 mM induced a transient and a sustained [Ca2+]i increase, respectively. The former, but not the latter, was inhibited by U-73122, indicating that the former was via the P2Y2 receptor and the latter via the P2X7 receptor. When external Cl- was replaced by other anions, the [Ca2+]i increase mediated by the P2Y2 receptor was not changed, but that mediated by the P2X7 receptor varied in the order of aspartate- > methanesulfonate > Cl- > Br > or = I-. In PC12 cells, transient [Ca2+]i increases mediated by the P2Y2 and P2X2 receptors were not affected by various anions. These results suggest that modulation by anions is unique to the P2X7 receptor and does not occur in P2Y2 and P2X2 receptors. This may be because the mechanism of ATP binding to the P2X7 receptor may be different than that to other P2 receptors.","PeriodicalId":14750,"journal":{"name":"Japanese journal of pharmacology","volume":"78 1","pages":"302-8"},"PeriodicalIF":0.0000,"publicationDate":"2002-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"12","resultStr":"{\"title\":\"Effects of anions on ATP-induced [Ca2+], increase in NG108-15 cells.\",\"authors\":\"T. Watano, I. Matsuoka, K. Ogawa, J. Kimura\",\"doi\":\"10.1254/JJP.89.302\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"We investigated the effects of anions on different P2 receptors by measuring ATP-induced increase in intracellular Ca2+ concentration ([Ca2+]i) in fura-2-loaded NG108-15 and PC12 cells. In NG108-15 cells, ATP at 100 microM and 1 mM induced a transient and a sustained [Ca2+]i increase, respectively. The former, but not the latter, was inhibited by U-73122, indicating that the former was via the P2Y2 receptor and the latter via the P2X7 receptor. When external Cl- was replaced by other anions, the [Ca2+]i increase mediated by the P2Y2 receptor was not changed, but that mediated by the P2X7 receptor varied in the order of aspartate- > methanesulfonate > Cl- > Br > or = I-. In PC12 cells, transient [Ca2+]i increases mediated by the P2Y2 and P2X2 receptors were not affected by various anions. These results suggest that modulation by anions is unique to the P2X7 receptor and does not occur in P2Y2 and P2X2 receptors. This may be because the mechanism of ATP binding to the P2X7 receptor may be different than that to other P2 receptors.\",\"PeriodicalId\":14750,\"journal\":{\"name\":\"Japanese journal of pharmacology\",\"volume\":\"78 1\",\"pages\":\"302-8\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2002-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"12\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Japanese journal of pharmacology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1254/JJP.89.302\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Japanese journal of pharmacology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1254/JJP.89.302","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 12
摘要
我们通过测量atp诱导的fura-2负载的NG108-15和PC12细胞内Ca2+浓度([Ca2+]i)的增加来研究阴离子对不同P2受体的影响。在NG108-15细胞中，100微米和1毫米ATP分别诱导瞬时和持续的[Ca2+]i升高。U-73122对前者有抑制作用，而对后者无抑制作用，说明前者是通过P2Y2受体，后者是通过P2X7受体。当外源Cl-被其他阴离子取代时，P2Y2受体介导的[Ca2+]i升高没有变化，而P2X7受体介导的[Ca2+]i升高的顺序为天冬氨酸- >甲磺酸- > Cl- > Br > or = i -。在PC12细胞中，P2Y2和P2X2受体介导的瞬时[Ca2+]i升高不受各种阴离子的影响。这些结果表明阴离子的调节是P2X7受体所特有的，而不发生在P2Y2和P2X2受体中。这可能是因为ATP与P2X7受体的结合机制可能不同于与其他P2受体的结合机制。
Effects of anions on ATP-induced [Ca2+], increase in NG108-15 cells.
We investigated the effects of anions on different P2 receptors by measuring ATP-induced increase in intracellular Ca2+ concentration ([Ca2+]i) in fura-2-loaded NG108-15 and PC12 cells. In NG108-15 cells, ATP at 100 microM and 1 mM induced a transient and a sustained [Ca2+]i increase, respectively. The former, but not the latter, was inhibited by U-73122, indicating that the former was via the P2Y2 receptor and the latter via the P2X7 receptor. When external Cl- was replaced by other anions, the [Ca2+]i increase mediated by the P2Y2 receptor was not changed, but that mediated by the P2X7 receptor varied in the order of aspartate- > methanesulfonate > Cl- > Br > or = I-. In PC12 cells, transient [Ca2+]i increases mediated by the P2Y2 and P2X2 receptors were not affected by various anions. These results suggest that modulation by anions is unique to the P2X7 receptor and does not occur in P2Y2 and P2X2 receptors. This may be because the mechanism of ATP binding to the P2X7 receptor may be different than that to other P2 receptors.
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