日本不同地区马铃薯叶片中粉色兼性甲基营养体的分布

IF 0.3 4区农林科学 Q4 AGRICULTURE, MULTIDISCIPLINARY

Jarq-japan Agricultural Research Quarterly Pub Date : 2021-10-01 DOI:10.6090/jarq.55.341

N. Someya, M. Kubota, Y. Unno, T. Morohoshi

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引用次数: 0

摘要

在日本的北海道、山形、茨城县、广岛和长崎五个地方种植了马铃薯(Solanum tuberosum)种子块茎。从马铃薯花期叶片中分离到100个粉红色兼性甲基营养体菌落。对分离株进行16S rRNA基因序列测定，聚类分析(一致性≥99%)鉴定出11个操作分类单元(otu)。系统发育分析表明，所有OTUs均归属于甲基杆菌属。OTU M9分布在所有5个收集点，其他OTU分布在几个收集点。这些数据表明，PPFMs在马铃薯层圈内的分布随植物地理位置的不同而不同。附加关键词:16S rRNA，甲基细菌，操作分类单位，种块茎

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Distribution of Pink-pigmented Facultative Methylotrophs Isolated from the Leaves of Potato Grown in Different Regions of Japan

Seed tubers of potato (Solanum tuberosum) plants were grown in five locations in Japan: Hokkaido, Yamagata, Ibaraki, Hiroshima, and Nagasaki. One hundred colonies of pink-pigmented facultative methylotrophs (PPFMs) were isolated from potato leaves at flowering time. The 16S rRNA gene sequences of isolates were determined, and clustering analysis (≥ 99% identity) identified 11 operational taxonomic units (OTUs). Phylogenetic analysis revealed that all OTUs were grouped in genus Methylobacterium. The OTU M9 was distributed among all five collection sites, and other OTUs were distributed among several collection sites. These data indicated that the distribution of PPFMs in the potato phyllosphere differed according to the plant’s geographical location. Discipline: Agricultural Environment Additional key words: 16S rRNA, Methylobacterium, operational taxonomic units, seed tuber

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来源期刊

Jarq-japan Agricultural Research Quarterly 农林科学-农业综合

CiteScore

1.00

自引率

25.00%

发文量

审稿时长

>36 weeks

期刊介绍： The Japan Agricultural Research Quarterly (JARQ) is a publication of the Japan International Research Center for Agricultural Sciences (JIRCAS), which provides readers overseas with the latest information on key achievements and developments in agricultural research in Japan, with the expectation that this information would contribute to the agricultural development of countries in tropical and subtropical regions.