海参不同上皮组织对光的行为反应及光相关基因Pax6的差异表达

IF 0.9 4区生物学 Q3 MARINE & FRESHWATER BIOLOGY

Marine and Freshwater Behaviour and Physiology Pub Date : 2020-03-03 DOI:10.1080/10236244.2020.1767505

Xiaolu Liu, Lin Chenggang, Lina Sun, Shilin Liu, Jing-chun Sun, Hongsheng Yang

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引用次数: 2

摘要

摘要本研究研究了光照条件下海参(Apostichopus japonicus)上皮组织的行为特征。行为学结果表明，触角、管足和刺对光敏感，结构发生变化。3种上皮组织中，触须最敏感，反应和恢复时间最短，而管足和棘的反应时间较长。采用实时荧光定量PCR (Quantitative real-time PCR, q-PCR)分析光照前后触手、管足和棘中Pax6的表达变化。Q-PCR结果显示，光照后上述3种组织中Pax6的表达量均高于光照前。我们假设海参可能没有独立的光感受器，但上皮组织能够表现出对光的行为反应。这将使我们能够重新审视海参所有上皮组织的功能。

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Behavioural response of different epithelial tissues of sea cucumber (Apostichopus japonicus) to light and differential expression of the light-related gene Pax6

ABSTRACT In this study, we evaluated the behavioural characteristics of the epithelial tissues of sea cucumber (Apostichopus japonicus) subjected to illumination. The behavioural results indicated that the tentacles, tube feet, and spines were sensitive to light with structural changes. Among the three epithelial tissues, the tentacles were the most sensitive with the shortest reaction and recovery times, whereas the tube feet and spines had longer reaction times. Quantitative real-time PCR (q-PCR) was used to analyse the changes of Pax6 expressions before and after illumination in the tentacles, tube feet and spines. Q-PCR results indicated that Pax6 expressions in the three above tissues after illumination were higher than that before illumination. We hypothesised that sea cucumber may not have independent photoreceptors, but the epithelial tissues were capable of exhibiting a behavioural response to light. This will allow us to re-examine the functions of all epithelial tissues of sea cucumbers.

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来源期刊

Marine and Freshwater Behaviour and Physiology 生物-海洋与淡水生物学

CiteScore

2.10

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Marine and Freshwater Behaviour and Physiology is devoted to the publication of papers covering field and laboratory research into all aspects of the behaviour and physiology of all marine and freshwater animals within the contexts of ecology, evolution and conservation. As the living resources of the world’s oceans, rivers and lakes are attracting increasing attention as food sources for humans and for their role in global ecology, the journal will also publish the results of research in the areas of fisheries biology and technology where the behaviour and physiology described have clear links to the contexts mentioned above. The journal will accept for publication Research Articles, Reviews, Rapid Communications and Technical Notes (see Instructions for authors for details). In addition, Editorials, Opinions and Book Reviews (invited and suggested) will also occasionally be published. Suggestions to the Editor-In-Chief for Special Issues are encouraged and will be considered on an ad hoc basis. With the goal of supporting early career researchers, the journal particularly invites submissions from graduate students and post-doctoral researchers. In addition to recognising the time constraints and logistical limitations their research often faces, and their particular need for a prompt review process, accepted articles by such researchers will be given prominence within the journal (see Instructions for authors for details).