Computational annotation of protein function

The term biological function of a protein is a little bit ambiguous. The exact meaning of this term varies and depends upon the context in which it is used. The term protein function is a broader term 3 and has more than one aspect. There are several computer-based tools which can be used for in silico prediction of protein function just out of protein sequence. While using a protein function prediction tool, it is important to consider which functional aspect is to be predicted.4