添加不同浓度葡萄糖的柠檬酸蛋黄水泥中长白猪精液的质量

Albertus Baku, A. A. Dethan, Paulus Klau Tahuk
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摘要

本研究的目的是研究添加不同浓度葡萄糖的柠檬酸蛋黄稀释液对长白猪水泥的运动性、活力、畸形和pH值的影响。所使用的水泥是从一头3岁长白种猪身上提取的新鲜水泥。本研究采用完全随机设计(CRD)的试验方法,4个处理(P1 5%, P2 10%, P3 15%, P4 20%), 4个重复,共16个试验单元。结果表明,卵黄柠檬酸盐稀释液中葡萄糖浓度对精子活力的影响不显著(P>0.05),各处理的平均值分别为P1(65%)、P2(60%)、P3(63,8%)和P4(67.5%)。精子存活率分别为P1(8.2%)、P2(9.2%)、P3(8.1%)和P4(9.2%),差异有统计学意义(P0.05)。各处理间pH值差异显著(P<0.05),其中P1处理与P2处理相对相同,但高于P4处理。另一方面,P3处理与P1、P2、P4处理相对相同,各处理的数值分别为P1(8.36)、P2(7.99)、P3(7.676)、P4(6.83)。由此可见,使用不同葡萄糖浓度的卵黄柠檬酸盐稀释液质量较好,即在葡萄糖浓度为15%的P3处理下,个体运动率平均为64%,活精子率为93.5%,异常精子率为8.1%。水泥的平均pH为7.68。综上所述,蛋黄柠檬酸盐稀释液中葡萄糖浓度为15%时,能较好地提高长白猪精液质量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Quality of Landrace Semen in Yolk Citrate Cement which Plus Glucose with Different Concentrations
The purpose of this study was to determine the effect of using yolk citrate diluent with different concentrations of glucose added on the motility, viability, abnormalities and pH of landrace pig cement.The cement used was fresh cement from a 3 year old Landrace pig stud.This study used an experimental method using a completely randomized design (CRD) with four treatments (P1 5%, P2 10%, P3 15% and P4 20%) and four replications so that there were 16 experimental units.The results showed that the concentration of glucose in the yolk citrate diluent was not significantly different (P>0.05) on the motility of spermatozoa with the average value of each treatment being P1 (65%), P2 (60%), P3 (63, 8%) and P4 (67.5%).The percentage of spermatozoa viability was very significantly different (P<0.01) between treatments, where in treatment P2 was relatively the same as treatment P3 and P4 but higher than treatment P1 with the value of each treatment P1 (83%), P2 (90.1 %), P3 (93.5%) and P4 (91.5%). Percentage of spermatozoa abnormalities resulting from analysis of variance (ANOVA) from each treatment there was a non-significant difference (P>0.05) where the values ​​were P1 (8.2%), P2 (9.2%), P3 (8.1%) and P4 (9.2%). The pH was significantly different (P<0.05) between treatments, where P1 treatment was relatively the same as P2 but higher than P4 treatment. On the other hand, treatment P3 was relatively the same as treatment P1, P2 and P4, with the values ​​of each treatment being P1 (8.36), P2 (7.99), P3 (7.676) and P4 (6.83). It can be concluded that the use of different glucose concentrations in the egg yolk citrate diluent with good quality, namely in the P3 treatment with a glucose concentration of 15%, the average percentage of individual motility was 64%, the percentage of live spermatozoa was 93.5%, the percentage of abnormal spermatozoa was 8.1%. and the average pH of cement is 7.68. It was concluded that the quality semen of Landrace pig could be improved optimally using glucose concentration in egg yolk citrate diluent  by 15%.
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