基于组织培养细胞分裂素差异的40GY伽玛射线辐照下pH 3.4适应蓝鲑的形态特征

I. Prihantoro, A. Anandia, A. Aryanto, P. Karti
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引用次数: 1

摘要

Lamtoro是一种蛋白质含量高的豆科乔木植物,对干燥胁迫有广泛的环境适应,但对酸性胁迫的适应相对有限。通过40 Gy γ射线辐照对愈伤组织进行诱变,产生适应pH 3.4的lamtoro愈伤组织候选者。本研究的目的是根据组织培养中细胞分裂素的差异,测定40Gy γ射线辐照后pH 3.4的适应lamtoro cv Tarramba的形态特征。本研究采用完全随机设计,采用动素、BAP和TDZ三种不同来源的细胞因子治疗,共10个重复。结果表明:在愈伤组织直径方面,0.5 ppm TDZ处理效果最好,愈伤组织质地疏松,但愈伤组织颜色反应倾向于淡绿色;
本文章由计算机程序翻译,如有差异,请以英文原文为准。
THE MORPHOLOGICAL CHARACTERISTICS OF ADAPTED LAMTORO (Leucaena leucocephala CV TARRAMBA) pH 3.4 THAT PRODUCED BY 40GY GAMMA RAY IRRADIATION BASED ON DIFFERENCES OF CYTOKININS IN TISSUE CULTURE
Lamtoro, a legume tree plant with high protein content that has a wide range of environmental adaptation to dry stress, but relatively limited to acid stress. Mutation of lamtoro through 40 Gy gamma ray irradiation on callus culture produces lamtoro callus candidates adapted with pH 3.4. Aim of the study was to measure the morphological characteristics of adapted lamtoro cv Tarramba pH 3.4 that produced by 40Gy gamma ray irradiation based on differences of cytokinins in tissue culture. The study used complete randomized design with three treatments of different sources of cytokines i.e. kinetin, BAP and TDZ with 10 replications. The results showed that 0.5 ppm TDZ was the best treatment in callus diameter, and produced crumbly callus texture, but showed callus color response that tended to be light green.
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