基因X两个三重突变在印尼西苏门答腊岛巴东地区慢性乙型肝炎病毒中的优势

Afida Razuna Ave, A. Putra, Saptino Miro
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引用次数: 0

摘要

背景:乙型肝炎是世界范围内发病率较高的主要健康问题。乙型肝炎是由乙型肝炎病毒引起的一种肝脏感染。慢性乙型肝炎是一种持续6个月以上的肝脏炎症,有可能发展为肝硬化和肝细胞癌。该疾病受X基因和病毒基因型的影响。基因X的突变被怀疑在疾病进展中起作用。本研究旨在检测印尼西苏门答腊巴东地区慢性乙型肝炎(CHB)临床样本中HBV基因X多态性和系统发育。方法:选取全部慢性hbv感染患者38例作为研究对象。研究样本为来自印度尼西亚红十字血库的全部乙肝血清,采用巢式PCR扩增基因X,得到两个片段,并与X序列数据库比对,继续进行突变分析。结果:本研究发现所有样本均存在核苷酸变异。在各种突变中,我们观察到已知肝硬化和hcc相关HBx蛋白突变的存在,即双突变(HBx130和HBx131)和两种三重突变(HBx5/HBx130/HBx131)和(HBx127/HBx130/HBx131)很高。分析还显示,患者主要感染基因型C,占72.2%,其次是基因型B,占27.8%。结论:所有样本均存在核苷酸变异,该变异提示慢性感染患者病毒与宿主之间的分子进展。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Gene X Two Triple Mutations Predominance on Chronic Hepatitis B Virus in Padang, West Sumatra Indonesia
Background: Hepatitis B is a health issue that become major problem worldwide with high morbidity. Hepatitis B is a liver infection that caused by Hepatitis B Virus. Chronic hepatitis B is a liver inflammation that lasted more than 6 months and it has the potential to progress to liver cirrhosis and hepatocellular cancer. The disease is influenced by Gene X and viral genotype. Mutations in the Gene X are suspected to having a role in disease progression. The aim of this study was to detect Gene X polymorphism and the phylogeny of HBV from Padang local clinical samples of chronic hepatitis B (CHB), West Sumatera, Indonesia.Method: The entire chronically HBV-infected patients were enrolled in this study: 38 CHB. The research samples were the entire Hepatitis B serum from Indonesian Red Cross Blood Bank than Gene X was amplified using nested PCR, which produced two fragments and aligned with X sequence database continued with mutation analysis. Results: In this study we found all the samples were having nucleotide variation. Of various mutations, we observed the presence of known liver cirrhosis and HCC-related HBx protein mutant i.e double mutations (HBx130 and HBx131) and two triple mutations (HBx5/HBx130/HBx131) and (HBx127/HBx130/HBx131) were high. The analysis also showed that patients were infected mainly by genotype C at 72,2% and followed by B at 27,8%.Conclusion: We conclude that all the samples have nucleotide variation and the mutation implying that molecular progression between the virus and the host at chronically infected patients.
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