水稻过氧化物酶编码序列的RFLP定位:与同工酶分析确定的位点不同的检测

The Japanese Journal of Genetics Pub Date : 1994-04-25 DOI:10.1266/JJG.69.101

N. Kishimoto, Hiroyuki Ito, Y. Ohashi, A. Saito

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摘要

利用籼稻(‘Kasalath’)与粳稻(‘FL134’)杂交的F2群体，对克隆的水稻过氧化物酶基因进行了RFLP定位。该过氧化物酶基因定位在3号染色体chl-1相对远端的两个RFLP标记(XNpb373和XNpb244-2)之间。结果表明，该过氧化物酶基因位点与同工酶分析确定的位点不同。

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RFLP mapping of a coding sequence for rice peroxidase: detection of a locus different from those determined by isozyme analyses

RFLP mapping of a cloned peroxidase gene from rice was performed using a F2 population derived from a crossing of an indica ('Kasalath') × a japonica ('FL134'). The peroxidase gene was mapped between two RFLP markers (XNpb373 and XNpb244-2) in the relatively distal region opposite to chl-1 on chromosome 3. It is concluded that this locus of the peroxidase gene is different from the loci determined by isozyme analyses.

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The Japanese Journal of Genetics

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