F. Umeda, T. Nishikawa, H. Miyasaka, I. Maeda, M. Kawase, Kiyohito Yagγ
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引用次数: 0
摘要
最近，我们从金黄色假单胞菌中克隆并分析了两个聚羟基烷酸酯(PHA)合成酶基因(PHA簇中的phaC1和phaC2)。本文将金黄色葡萄球菌的PHA合成酶1和PHA合成酶2的氨基酸序列与含有同源PHA簇的假单胞菌(Pseudomonas sp. 61-3, P. oleovorans和P. aeruginosa)进行了比较。三种菌株的PHA合成酶1和PHA合成酶2的同源性均较高。此外，PHA合成酶AA序列的多重比对表明，PHA合成酶1和PHA合成酶2在包括金黄色葡萄球菌在内的4株菌株中均高度保守。
Short Communication: Homology Study of Two Polyhydroxyalkanoate (PHA) Synthases from Pseudomonas Aureofaciens
Recently, we have cloned and analyzed two polyhydroxyalkanoate (PHA) synthase genes (phaC1 and phaC2 in the pha cluster) from Pseudomonas aureofaciens. In this report, the deduced amino acid (AA) sequences of PHA synthase 1 and PHA synthase 2 from P. aureofaciens are compared with those from three other bacterial strains (Pseudomonas sp. 61–3, P. oleovorans and P. aeruginosa) containing the homologous pha cluster. The level of homology of either PHA synthase 1 or PHA synthase 2 was high with each enzyme from these three bacterial strains. Furthermore, multialignment of PHA synthase AA sequences implied that both enzymes of PHA synthase 1 and PHA synthase 2 were highly conserved in the four strains including P. aureofaciens.
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