呼吸道感染患者肠道病毒的遗传多样性

N. Paklonskaya, T. Amvrosieva, Y. Shilova, E. Kishkurno
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引用次数: 0

摘要

肠道病毒的遗传变异是其引起的各种临床疾病的基础。本研究的目的是确定2016-2019年引起急性呼吸道感染(ARI)的肠病毒(ev)的遗传多样性。对203例不同类型ARI患者采集生物标本,采用RT-PCR进行EV检测,并对主要衣壳蛋白基因进行测序和系统发育重建。34.4%的样本中检测到EV RNA,最常见于1-6岁儿童(53.1 - 54.8%)。柯萨奇病毒B型在呼吸道肠道病毒感染(EVI)患者中的检出率明显高于其他EVI,主要血清型为柯萨奇病毒B4、B5。尽管在ARI患者中发现的EVs具有显著的遗传多样性(柯萨奇病毒B5的3个遗传系、柯萨奇病毒B2的2个基因型、柯萨奇病毒B3的1个基因型、柯萨奇病毒B4的3个基因变异型、柯萨奇病毒B1的1个基因变异型),但没有证据表明它们与呼吸道EVI的形成有关。ev的高度遗传变异性要求定期进行分子流行病学监测,以识别新出现的遗传变异并评估其流行潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Genetic diversity of enteroviruses in patients with respiratory infection
Enterovirus genetic variability underlies the variety of clinical forms of diseases they cause. The aim of the presented study was to establish the genetic diversity of enteroviruses (EVs) that caused acute respiratory infection (ARI) in 2016–2019. Biological samples were obtained from 203 patients with various forms of ARI, EV detection was carried out by RT-PCR, followed by sequencing of the main capsid protein gene and phylogenetic reconstruction. EV RNA was detected in 34.4 % of samples, most often in children aged 1–6 years (53.1–54.8 %). Coxsackieviruses B were found in patients with respiratory enterovirus infection (EVI) significantly more often than other EVs, the dominant serotypes were Coxsackievirus B4, B5. Despite the significant genetic diversity of EVs identified in patients with ARI (three genetic lines of Coxsackievirus B5, two genotypes of Coxsackievirus B2, one genotype of Coxsackievirus B3, three genovariant Coxsackievirus B4, one genovariant Coxsackievirus B1), there is no evidence of their connection with the formation of the respiratory form of EVI.The high level of genetic variability of EVs requires regular molecular-epidemiological surveillance for the identification of emerging genetic variants and assessment of their epidemic potential.
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