{"title":"间接免疫荧光法分析大豆染色体复制模式","authors":"T. Yanagisawa, S. Tano, K. Fukui, K. Harada","doi":"10.1266/JJG.68.119","DOIUrl":null,"url":null,"abstract":"An indirect immunofluorescence method detecting the incorporation of BrdU was applied to analyze the DNA replication pattern in soybean chromosomes which are very small among plant species so far examined. Discrete fluorescent images in the distal, interstitial and proximal regions of the chromosome arms were revealed at prometaphase. Time-course analysis indicated that the DNA replication starts at the distal region. The replication patterns are discussed in relation to other chromosomal characteristics such as the location of constitutive heterochromatin and the stainability with the Giemsa.","PeriodicalId":22578,"journal":{"name":"The Japanese Journal of Genetics","volume":"310 1","pages":"119-125"},"PeriodicalIF":0.0000,"publicationDate":"1993-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"4","resultStr":"{\"title\":\"Analysis of replication pattern in soybean chromosomes by indirect immunofluorescence method\",\"authors\":\"T. Yanagisawa, S. Tano, K. Fukui, K. Harada\",\"doi\":\"10.1266/JJG.68.119\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"An indirect immunofluorescence method detecting the incorporation of BrdU was applied to analyze the DNA replication pattern in soybean chromosomes which are very small among plant species so far examined. Discrete fluorescent images in the distal, interstitial and proximal regions of the chromosome arms were revealed at prometaphase. Time-course analysis indicated that the DNA replication starts at the distal region. The replication patterns are discussed in relation to other chromosomal characteristics such as the location of constitutive heterochromatin and the stainability with the Giemsa.\",\"PeriodicalId\":22578,\"journal\":{\"name\":\"The Japanese Journal of Genetics\",\"volume\":\"310 1\",\"pages\":\"119-125\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1993-04-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Japanese Journal of Genetics\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1266/JJG.68.119\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Japanese Journal of Genetics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1266/JJG.68.119","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Analysis of replication pattern in soybean chromosomes by indirect immunofluorescence method
An indirect immunofluorescence method detecting the incorporation of BrdU was applied to analyze the DNA replication pattern in soybean chromosomes which are very small among plant species so far examined. Discrete fluorescent images in the distal, interstitial and proximal regions of the chromosome arms were revealed at prometaphase. Time-course analysis indicated that the DNA replication starts at the distal region. The replication patterns are discussed in relation to other chromosomal characteristics such as the location of constitutive heterochromatin and the stainability with the Giemsa.
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