从猪肉生产链不同阶段分离的植物乳杆菌对大肠弯曲杆菌的抑制作用

M. J. Ruiz
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引用次数: 0

摘要

植物乳杆菌对环境的适应能力强,对不同的病原菌均有较强的抑菌能力。这是一种潜在的替代方法,可用于食源性屠宰动物病原体的控制。研究植物乳杆菌对大肠杆菌的抑菌作用。采用猪源植物乳杆菌LP1、LP3、LP5和LP7和大肠弯曲杆菌NCTC 11366作为对照菌株。大肠杆菌和植物乳杆菌被重新激活。冻干后得到ELC和ELCn。用棉签将C. coli分布在mCCDA表面,将ELC和ELCn接种于事先在琼脂上打好的孔中。48 h后,测定抑制区直径。产生的抑制光晕为23.2 mm。ELCn表现出较少的拮抗活性。LP5 ELC产生较高的抑制光晕,ELCn在4株菌株之间无差异。从猪生产链中分离出的植物乳杆菌菌株可能用于控制大肠杆菌。这一数据补充了在这种对公共卫生具有重要意义的人畜共患病原体之前,植物芽孢杆菌抑制作用的缺乏科学证据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
EFECTO INHIBITORIO DE Lactobacillus plantarum AISLADOS DESDE LAS DISTINTAS ETAPAS DE LA CADENA PRODUCTIVA DE CARNE PORCINA FRENTE A Campylobacter coli
Lactobacillus plantarum has a great capacity to adapt to environmental and have antibacterial capacity against different pathogen. This is a potential alternative for the control of pathogen in food coming slaughter animal. The objective of this work was to determine the antibacterial effect of L. plantarum strains against C. coli strains. L. plantarum LP1, LP3, LP5 and LP7 of porcine origin and a reference strain of Campylobacter coli NCTC 11366 were used. C. coli and L. plantarum was reactivated. Lyophilized ELC and ELCn were obtained. C. coli was distributed on the surface of mCCDA by swab and the ELC and the ELCn were inoculated in wells previously made on the agar. After 48 h, the diameter of the zone of inhibition was measured. The inhibition halos produced were 23.2 mm. The ELCn showed less antagonistic activity. The LP5 ELC generated higher inhibition halos and ELCn showed no differences between the four strains studied. L. plantarum strains, isolated from the pig production chain, could potentially be applied to control C. coli. This data is added to the scarce scientific evidence of the inhibitory effect of L. plantarum before this zoonotic pathogen of importance in public health.
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