加瓦尔喜马拉雅地区法国豆种质抗角斑病评价

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-03-08 DOI:10.1017/s1479262123000126

Navneeti Chamoli, D. Prabha, Y. Negi, J. Chauhan

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引用次数: 0

摘要

角斑叶斑病是由灰豆伪cercospora grisiseola引起的一种世界性的主要病害。在印度北阿坎德邦的加尔瓦尔喜马拉雅山脉，存在着多种多样的法国豆，这是尚未开发的。本研究的目的是鉴定该地区法国豆地方品种的抗als遗传。从北阿坎德邦加尔瓦尔的不同村庄收集了176名当地成员。通过4条SCAR引物SN02 (Phg-2)、SAA19、SM02、SBA16 (Phg-3)、1条STS引物TGA1.1 (Phg-1)和1条SSR引物Pv-at006 (Phg-5)进行筛选。并在2019年和2020年对所有材料进行田间条件下的ALS抗性筛选。按1-9分进行抗病评分。田间筛选后，筛选48份材料(抗性19份，中等抗性24份，敏感5份)，在筛房条件下进行体外筛选。采用北阿坎德邦特赫里林业学院微生物实验室鉴定的两种不同病原菌P5和P9进行人工接种。经体外筛选，7个菌株(ggb -25、ggb -26、ggb -30、ggb -32、ggb -93、ggb -97和ggb -136)对P5和P9菌株均有抗性。在未来的育种计划中，可以进一步利用抗灰斑病的材料来开发抗ALS的法国豆新品种。

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Evaluation of French bean germplasm from Garhwal Himalayas for resistance to angular leaf spot

Angular leaf spot (ALS) caused by Pseudocercospora griseola is a major disease of french bean (Phaseolus vulgaris L.) worldwide. A good diversity of French bean is present in the Garhwal Himalayas of Uttarakhand, India, which is unexplored. The purpose of this study was to identify ALS-resistant accessions among local landraces of French bean in this region. One hundred seventy-six local accessions were collected from different villages of Garhwal, Uttarakhand. All the accessions were screened by four SCAR primers SN02 (Phg-2), SAA19, SM02, SBA16 (Phg-3), one STS primer TGA1.1 (Phg-1) and one SSR primer Pv-at006 (Phg-5). All the accessions were also screened for ALS resistance under field condition in the years 2019 and 2020. The disease-resistant score was recorded on 1–9 scale. After field screening, 48 accessions (19 resistant, 24 moderately resistant and five susceptible) were selected for in-vitro screening under screen house condition. These 46 accessions were artificially inoculated by two different isolates of P. griseola P5 and P9, which are the most virulent pathotype characterized by microbiology lab, College of Forestry, Tehri, Uttarakhand. After in-vitro screening, seven accessions (GFB-25, GFB-26, GFB-30, GFB-32, GFB-93, GFB-97 and GFB-136) were found resistant to both the isolates P5 and P9. The P. griseola-resistant accessions may further be used in future breeding programmes to develop new and more resistant varieties of French bean against ALS.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.