蚂蚁染色体制备(改进的风干法)和吉姆萨染色手册

H. Imai
{"title":"蚂蚁染色体制备(改进的风干法)和吉姆萨染色手册","authors":"H. Imai","doi":"10.11352/SCR.19.57","DOIUrl":null,"url":null,"abstract":"I. CHEMICALS (1) Colchicine stock solution (1mg/ml, 0.1% solution) (a) 50 mg colchicine (b) 50 ml distilled water (D.W.) * Store in a brown bottle and keep in a refrigerator. (2) Hypotonic solution (1% Sodium citrate solution) (a) 1 g Trisodium citrate dihydrate (b) 100 ml D.W. (3) Colchicine-hypotonic solution (* freshly prepared) 10 ml Hypotonic solution with 0.005 % colchicine (a) 0.5 ml Colchicine stock solution (b) 9.5 ml Hypotonic solution (4) Fixative I (* freshly prepared) 60% 1:1 Acetic-ethanol (a) Glacial acetic acid 3 ml (b) Ethanol (>99.5%) 3 ml (c) D.W. 4 ml * Don’t store Fixative I overnight, because it turns rapidly into ethyl acetate, which is deleterious to mitotic cells. * Don’t use old glacial acetic acid and ethanol that are near the bottom of a stock bottle or that have been left for several months. Both chemicals tend to absorb moisture from the air and chromosomes will be damaged by the contamination of water. (5) Fixative II (* freshly prepared) 4 ml Absolute 1:1 Acetic-ethanol (a) Glacial acetic acid 2 ml (b) Ethanol (>99.5%) 2 ml (6) Fixative III 2ml Glacial acetic acid (GAA) II. APPARATUS (Fig. 1) (1) A transmitted stereomicroscope (x20-x40) for dissecting out organs (Fig. 1a). (2) A pair of dissecting needles (Fig. 1b). (3) Four Pasteur pipettes each with a rubber nipple (Fig. 1c). (4) Centrifugation tube (Spits tube) (Fig. 1d). (5) Depression slides (hole slides) (Fig. 1e). (6) Slide glasses (Fig. 1f). * Store slides in 80% ethanol and dry with washed clean gauze as necessary. * If slides are oily, add a drop of fixative I on the slide, and clear with paper or washed clean gauze. (7) Test tube stand (Fig. 1g). (8) Filter paper (blotting paper) (Fig. 1h). (9) Rolled filter paper or paper towel (Fig. 1i). (10) Fingerstalls for the thumb and the forefinger (Fig. 1j). * To protect fingers from fixative. * Cut the tip of fingers of a vinyl glove for right hand. (11) Washed cotton gauze or tissue paper (Fig. 1k). (12) A glass engraving pen for label (Fig. 1l). (13) Forceps to open cocoons (Fig. 1m).","PeriodicalId":10221,"journal":{"name":"Chromosome science","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"4","resultStr":"{\"title\":\"A manual for ant chromosome preparations (an improved air-drying method) and Giemsa staining\",\"authors\":\"H. Imai\",\"doi\":\"10.11352/SCR.19.57\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"I. CHEMICALS (1) Colchicine stock solution (1mg/ml, 0.1% solution) (a) 50 mg colchicine (b) 50 ml distilled water (D.W.) * Store in a brown bottle and keep in a refrigerator. (2) Hypotonic solution (1% Sodium citrate solution) (a) 1 g Trisodium citrate dihydrate (b) 100 ml D.W. (3) Colchicine-hypotonic solution (* freshly prepared) 10 ml Hypotonic solution with 0.005 % colchicine (a) 0.5 ml Colchicine stock solution (b) 9.5 ml Hypotonic solution (4) Fixative I (* freshly prepared) 60% 1:1 Acetic-ethanol (a) Glacial acetic acid 3 ml (b) Ethanol (>99.5%) 3 ml (c) D.W. 4 ml * Don’t store Fixative I overnight, because it turns rapidly into ethyl acetate, which is deleterious to mitotic cells. * Don’t use old glacial acetic acid and ethanol that are near the bottom of a stock bottle or that have been left for several months. Both chemicals tend to absorb moisture from the air and chromosomes will be damaged by the contamination of water. (5) Fixative II (* freshly prepared) 4 ml Absolute 1:1 Acetic-ethanol (a) Glacial acetic acid 2 ml (b) Ethanol (>99.5%) 2 ml (6) Fixative III 2ml Glacial acetic acid (GAA) II. APPARATUS (Fig. 1) (1) A transmitted stereomicroscope (x20-x40) for dissecting out organs (Fig. 1a). (2) A pair of dissecting needles (Fig. 1b). (3) Four Pasteur pipettes each with a rubber nipple (Fig. 1c). (4) Centrifugation tube (Spits tube) (Fig. 1d). (5) Depression slides (hole slides) (Fig. 1e). (6) Slide glasses (Fig. 1f). * Store slides in 80% ethanol and dry with washed clean gauze as necessary. * If slides are oily, add a drop of fixative I on the slide, and clear with paper or washed clean gauze. (7) Test tube stand (Fig. 1g). (8) Filter paper (blotting paper) (Fig. 1h). (9) Rolled filter paper or paper towel (Fig. 1i). (10) Fingerstalls for the thumb and the forefinger (Fig. 1j). * To protect fingers from fixative. * Cut the tip of fingers of a vinyl glove for right hand. (11) Washed cotton gauze or tissue paper (Fig. 1k). (12) A glass engraving pen for label (Fig. 1l). (13) Forceps to open cocoons (Fig. 1m).\",\"PeriodicalId\":10221,\"journal\":{\"name\":\"Chromosome science\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2016-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chromosome science\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.11352/SCR.19.57\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chromosome science","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.11352/SCR.19.57","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 4

摘要

(1)秋水仙碱原液(1mg/ml, 0.1%溶液)(a) 50 mg秋水仙碱(b) 50 ml蒸馏水(D.W.) *保存于棕色瓶中,冰箱保存。(2)低渗的溶液(1%柠檬酸钠溶液)(a) 1 g柠檬酸三钠二水合物(b) 100毫升D.W. (3) Colchicine-hypotonic解决方案(*刚做好的)10毫升低渗的溶液与0.005%秋水仙碱(一)0.5毫升秋水仙素原液(b) 9.5毫升低渗的解决方案(4)固着我(*刚做好的)60% 1:1 Acetic-ethanol(一)冰醋酸3毫升乙醇(b)(> 99.5%) * 3毫升(c) D.W. 4毫升不存储固定剂我一夜之间,因为它迅速转化为乙酸乙酯,对有丝分裂细胞是有害的。*不要使用酒瓶底部或已存放数月的旧冰醋酸和乙醇。这两种化学物质都倾向于从空气中吸收水分,染色体会因水的污染而受损。(5)固定液II(*新鲜配制)4ml绝对1:1醋酸-乙醇(a)冰醋酸2ml (b)乙醇(>99.5%)2ml(6)固定液III 2ml冰醋酸(GAA) II。(1)用于解剖器官的透射体视显微镜(x20-x40)(图1a)。(2)一对解剖针(图1b)。(3)四个巴斯德移液管,每个移液管有一个橡胶接头(图1c)。(4)离心管(Spits管)(图1d)。(5)凹陷滑块(孔滑块)(图1e)。(6)载玻片(图1f)。*将载玻片存放在80%乙醇中,必要时用洗净的纱布擦干。*如果载玻片是油性的,在载玻片上加一滴固定液,然后用纸或洗净的纱布擦净。(7)试管架(图1g)。(8)滤纸(吸墨纸)(图1h)。(9)卷过滤纸或纸巾(图1i)。(10)拇指和食指指节(图1j)。*保护手指不被固定。*切掉右手乙烯基手套的指尖。(11)水洗棉纱布或薄纸(图1k)。(12)标签玻璃雕刻笔(图11)。(13)开茧钳(图1m)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A manual for ant chromosome preparations (an improved air-drying method) and Giemsa staining
I. CHEMICALS (1) Colchicine stock solution (1mg/ml, 0.1% solution) (a) 50 mg colchicine (b) 50 ml distilled water (D.W.) * Store in a brown bottle and keep in a refrigerator. (2) Hypotonic solution (1% Sodium citrate solution) (a) 1 g Trisodium citrate dihydrate (b) 100 ml D.W. (3) Colchicine-hypotonic solution (* freshly prepared) 10 ml Hypotonic solution with 0.005 % colchicine (a) 0.5 ml Colchicine stock solution (b) 9.5 ml Hypotonic solution (4) Fixative I (* freshly prepared) 60% 1:1 Acetic-ethanol (a) Glacial acetic acid 3 ml (b) Ethanol (>99.5%) 3 ml (c) D.W. 4 ml * Don’t store Fixative I overnight, because it turns rapidly into ethyl acetate, which is deleterious to mitotic cells. * Don’t use old glacial acetic acid and ethanol that are near the bottom of a stock bottle or that have been left for several months. Both chemicals tend to absorb moisture from the air and chromosomes will be damaged by the contamination of water. (5) Fixative II (* freshly prepared) 4 ml Absolute 1:1 Acetic-ethanol (a) Glacial acetic acid 2 ml (b) Ethanol (>99.5%) 2 ml (6) Fixative III 2ml Glacial acetic acid (GAA) II. APPARATUS (Fig. 1) (1) A transmitted stereomicroscope (x20-x40) for dissecting out organs (Fig. 1a). (2) A pair of dissecting needles (Fig. 1b). (3) Four Pasteur pipettes each with a rubber nipple (Fig. 1c). (4) Centrifugation tube (Spits tube) (Fig. 1d). (5) Depression slides (hole slides) (Fig. 1e). (6) Slide glasses (Fig. 1f). * Store slides in 80% ethanol and dry with washed clean gauze as necessary. * If slides are oily, add a drop of fixative I on the slide, and clear with paper or washed clean gauze. (7) Test tube stand (Fig. 1g). (8) Filter paper (blotting paper) (Fig. 1h). (9) Rolled filter paper or paper towel (Fig. 1i). (10) Fingerstalls for the thumb and the forefinger (Fig. 1j). * To protect fingers from fixative. * Cut the tip of fingers of a vinyl glove for right hand. (11) Washed cotton gauze or tissue paper (Fig. 1k). (12) A glass engraving pen for label (Fig. 1l). (13) Forceps to open cocoons (Fig. 1m).
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信