山李砧木胚培养及离体克隆增殖研究

IF 1.3 4区农林科学 Q3 AGRONOMY

Crop Breeding and Applied Biotechnology Pub Date : 2003-06-30 DOI:10.12702/1984-7033.V03N02A07

M. P. Guerra, M. Rogalski, Aparecido Lima da Silva

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引用次数: 1

摘要

胚胎培养和恢复是李属植物形态发生、离体繁殖和遗传育种的基础和应用研究技术。研究了不同浓度ga3培养基对‘Capdeboscq’砧木胚萌发和器官发育的影响，并对其无性系的体外增殖率进行了研究。取胚轴，接种于添加BAP (0.1 mg L -1)、NAA (0.1 mg L -1)和ga3 (0.0;1.0;2.5;5.0;7.5和10.0 mg L -1)。在最后一次传代培养中，采用双相增殖法测定了4个无性系胚萌发后的体外增殖潜力。结果表明，离体萌发率平均为70.7%。GA 3对种子萌发无明显影响，但能促进胚伸长，抑制根发育。在胚培养中获得的无性系具有较高的离体增殖潜力，每个外植体分别有6.9和14.9个芽。

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Embryo culture and in vitro clonal multiplication of Prunus 'Capdeboscq' rootstock

Embryo culture and recovery are techniques used in basic and applied studies of morphogenesis, in vitro propagation and genetic breeding in the Prunus genus. The present study assessed the germination and organogenetic development of embryos in culture media with different concentrations of GA 3 as well as the in vitro multiplication rate of clones of the ‘Capdeboscq’ rootstock. Embryonic axes were excised and inoculated in Lepoivre culture medium supplemented with BAP (0.1 mg L -1 ), NAA (0.1 mg L -1 ) and GA 3 (0.0; 1.0; 2.5; 5.0; 7.5 and 10.0 mg L -1 ). The in vitro multiplication potential of four clones was assessed after embryo germination using the double phase multiplication method in the last subculture. The results showed a mean of 70.7% in vitro germination. GA 3 was not effective in the germination, but induced greater elongation of the embryo and inhibited the root development. The clones obtained in the embryo culture showed high potential for in vitro multiplication with rates of 6.9 and 14.9 shoots per explant.

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来源期刊

Crop Breeding and Applied Biotechnology AGRONOMY-BIOTECHNOLOGY & APPLIED MICROBIOLOGY

CiteScore

2.70

自引率

13.30%

发文量

审稿时长

6-12 weeks

期刊介绍： The CBAB – CROP BREEDING AND APPLIED BIOTECHNOLOGY (ISSN 1984-7033) – is the official quarterly journal of the Brazilian Society of Plant Breeding, abbreviated CROP BREED APPL BIOTECHNOL. It publishes original scientific articles, which contribute to the scientific and technological development of plant breeding and agriculture. Articles should be to do with basic and applied research on improvement of perennial and annual plants, within the fields of genetics, conservation of germplasm, biotechnology, genomics, cytogenetics, experimental statistics, seeds, food quality, biotic and abiotic stress, and correlated areas. The article must be unpublished. Simultaneous submitting to another periodical is ruled out. Authors are held solely responsible for the opinions and ideas expressed, which do not necessarily reflect the view of the Editorial board. However, the Editorial board reserves the right to suggest or ask for any modifications required. The journal adopts the Ithenticate software for identification of plagiarism. Complete or partial reproduction of articles is permitted, provided the source is cited. All content of the journal, except where identified, is licensed under a Creative Commons attribution-type BY. All articles are published free of charge. This is an open access journal.