白藜芦醇对雄性成年大鼠顺铂睾丸毒性的影响

Dr. Shahnaz Memon, Dr. Sajjad Ali Almani, Dr. Muhammad Saqib Baloch, Dr. Samreen Ali, Tanveer Ahmed Talpur, Harender Kumar
{"title":"白藜芦醇对雄性成年大鼠顺铂睾丸毒性的影响","authors":"Dr. Shahnaz Memon, Dr. Sajjad Ali Almani, Dr. Muhammad Saqib Baloch, Dr. Samreen Ali, Tanveer Ahmed Talpur, Harender Kumar","doi":"10.35787/jimdc.v11i1.716","DOIUrl":null,"url":null,"abstract":"Background and Objective: Resveratrol is a poly-hydroxyphenol plant toxin that alleviates oxidative stress by increasing endogenous antioxidant levels and prevents tissue damage. The study aimed to investigate the anti-oxidative role of Resveratrol by histochemical, ultrastructural, and biochemical methods in Cisplatin-induced testicular toxicity in Wistar Albino rats. \nMaterial and Methods: Quasi-experimental study was conducted at the Department of Pharmacology, Anatomy and Postgraduate Laboratory of ISRA University Hyderabad from October 2020 to March 2021. Twenty-four male, normal adult Wistar albino rats were recruited and distributed equally into; Group-A (Control), Group-B (Experimental group or Cisplatin group), Group-C (Experimental group or Cisplatin + Resveratrol combination group). Pre and post-experimental body weight, analysis of oxidative markers, semen parameters, and histomorphology were carried out in all three groups. SPSS version 24.0 was used for the analysis of Data. \nResults: A statistically significant decline in the body weight and testicular weight in group B and C respectively (p<0.05). While reduction in sperm count, motility and viability was observed in Group-B compared with Group-C (p<0.05). Oxidative markers were also significantly depleted in Group-B in comparison to Group-C (p<0.05). Evident changes were observed in the testicular histology of Group-B compared with Group-C (p<0.05). Irregular, regressive, and atrophic seminiferous tubules were seen in Group-B. Most seminiferous tubules having normal morphology were observed in Group-C while the number of atrophic and degenerative seminiferous tubules also decreased significantly. \nConclusion: Resveratrol is a potent protective agent with promising results in attenuating cisplatin-induced oxidative stress and eventually testicular toxicity.","PeriodicalId":33701,"journal":{"name":"Journal of Islamabad Medical and Dental College","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2022-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Impact of Resveratrol in Attenuating Cisplatin Induced Testicular Toxicity in Male adult Rats\",\"authors\":\"Dr. Shahnaz Memon, Dr. Sajjad Ali Almani, Dr. Muhammad Saqib Baloch, Dr. Samreen Ali, Tanveer Ahmed Talpur, Harender Kumar\",\"doi\":\"10.35787/jimdc.v11i1.716\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Background and Objective: Resveratrol is a poly-hydroxyphenol plant toxin that alleviates oxidative stress by increasing endogenous antioxidant levels and prevents tissue damage. The study aimed to investigate the anti-oxidative role of Resveratrol by histochemical, ultrastructural, and biochemical methods in Cisplatin-induced testicular toxicity in Wistar Albino rats. \\nMaterial and Methods: Quasi-experimental study was conducted at the Department of Pharmacology, Anatomy and Postgraduate Laboratory of ISRA University Hyderabad from October 2020 to March 2021. Twenty-four male, normal adult Wistar albino rats were recruited and distributed equally into; Group-A (Control), Group-B (Experimental group or Cisplatin group), Group-C (Experimental group or Cisplatin + Resveratrol combination group). Pre and post-experimental body weight, analysis of oxidative markers, semen parameters, and histomorphology were carried out in all three groups. SPSS version 24.0 was used for the analysis of Data. \\nResults: A statistically significant decline in the body weight and testicular weight in group B and C respectively (p<0.05). While reduction in sperm count, motility and viability was observed in Group-B compared with Group-C (p<0.05). Oxidative markers were also significantly depleted in Group-B in comparison to Group-C (p<0.05). Evident changes were observed in the testicular histology of Group-B compared with Group-C (p<0.05). Irregular, regressive, and atrophic seminiferous tubules were seen in Group-B. Most seminiferous tubules having normal morphology were observed in Group-C while the number of atrophic and degenerative seminiferous tubules also decreased significantly. \\nConclusion: Resveratrol is a potent protective agent with promising results in attenuating cisplatin-induced oxidative stress and eventually testicular toxicity.\",\"PeriodicalId\":33701,\"journal\":{\"name\":\"Journal of Islamabad Medical and Dental College\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-03-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Islamabad Medical and Dental College\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.35787/jimdc.v11i1.716\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Islamabad Medical and Dental College","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.35787/jimdc.v11i1.716","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1

摘要

背景与目的:白藜芦醇是一种多羟基酚植物毒素,通过增加内源性抗氧化剂水平来缓解氧化应激,防止组织损伤。本研究旨在通过组织化学、超微结构和生化等方法探讨白藜芦醇在顺铂致Wistar白化大鼠睾丸毒性中的抗氧化作用。材料与方法:准实验研究于2020年10月至2021年3月在海德拉巴ISRA大学药理学、解剖学和研究生实验室进行。选取24只正常成年雄性Wistar白化大鼠,平均分布于;a组(对照组)、b组(实验组或顺铂组)、c组(实验组或顺铂+白藜芦醇联合组)。对三组大鼠进行实验前和实验后的体重、氧化标志物、精液参数和组织形态学分析。采用SPSS 24.0版本进行数据分析。结果:B、C组大鼠体重、睾丸重量下降,差异均有统计学意义(p<0.05)。与c组相比,b组精子数量、活力和活力均降低(p<0.05)。与c组相比,b组氧化标志物明显减少(p<0.05)。与c组比较,b组睾丸组织学变化明显(p<0.05)。b组精管不规则、退变、萎缩。c组大多数精管形态正常,而萎缩和退行性精管数量也明显减少。结论:白藜芦醇是一种有效的保护剂,在减轻顺铂诱导的氧化应激和最终睾丸毒性方面具有良好的效果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Impact of Resveratrol in Attenuating Cisplatin Induced Testicular Toxicity in Male adult Rats
Background and Objective: Resveratrol is a poly-hydroxyphenol plant toxin that alleviates oxidative stress by increasing endogenous antioxidant levels and prevents tissue damage. The study aimed to investigate the anti-oxidative role of Resveratrol by histochemical, ultrastructural, and biochemical methods in Cisplatin-induced testicular toxicity in Wistar Albino rats. Material and Methods: Quasi-experimental study was conducted at the Department of Pharmacology, Anatomy and Postgraduate Laboratory of ISRA University Hyderabad from October 2020 to March 2021. Twenty-four male, normal adult Wistar albino rats were recruited and distributed equally into; Group-A (Control), Group-B (Experimental group or Cisplatin group), Group-C (Experimental group or Cisplatin + Resveratrol combination group). Pre and post-experimental body weight, analysis of oxidative markers, semen parameters, and histomorphology were carried out in all three groups. SPSS version 24.0 was used for the analysis of Data. Results: A statistically significant decline in the body weight and testicular weight in group B and C respectively (p<0.05). While reduction in sperm count, motility and viability was observed in Group-B compared with Group-C (p<0.05). Oxidative markers were also significantly depleted in Group-B in comparison to Group-C (p<0.05). Evident changes were observed in the testicular histology of Group-B compared with Group-C (p<0.05). Irregular, regressive, and atrophic seminiferous tubules were seen in Group-B. Most seminiferous tubules having normal morphology were observed in Group-C while the number of atrophic and degenerative seminiferous tubules also decreased significantly. Conclusion: Resveratrol is a potent protective agent with promising results in attenuating cisplatin-induced oxidative stress and eventually testicular toxicity.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
35
审稿时长
16 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信