Total phenolic and flavonoid contents of n-hexane fraction in binjai leaves (Mangifera caesia Jack. ex. Wall)

Abstract Background: Binjai leaves (Mangifera caesia Jack. ex. Wall) or M. caesia contain phenolic and flavonoid compounds important in medicinal plants development. The solvent difference and fractionation will give various active substance concentrations. Objective: To evaluate the phenolic and flavonoid content qualitatively and quantitatively from the n-hexane fraction of binjai leaves methanol extract. Methods: The soxhletation method with methanol was used to extract binjai leaves followed by fractionation. Thin Layer Chromatography (TLC) followed by spraying reagents were used to test phenols and flavonoids compounds. Folin-Ciocalteu reagents were used to analyzed total phenolics, while AlCl3 reagents were used to quantify the total flavonoid content. Result: This study gave positive results on the qualitative test of phenolics and flavonoids. Meanwhile, the fraction assay results showed total phenolic levels of 45.19 µgGAE/mg and total flavonoid levels of 165.06 µgQE/mg. Conclusion: The n-hexane fraction of M. caesia leaves methanol extracts contains phenols and flavonoid content so that it has the potential as a medicinal plant. Keywords: Binjai leaves, methanol extracts, n-hexane fraction, phenol, flavonoid