适于茶树大田基因库的DNA条形码分析

茶叶科学 Pub Date : 2020-01-01 DOI:10.5376/JTSR.2020.10.0002

Yanyan Li, Wei Huang, Jia-Sian Lin, Chuanpeng Nie

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引用次数: 0

摘要

随着DNA条形码研究的深入，其应用越来越受到人们的重视。本研究以100棵茶树样品为研究对象，利用叶绿体matK和rbcL基因的部分序列对适合茶树的分子条形码进行了研究。结果表明，100棵茶树样品的rbcL序列相同，而matK序列不同，遗传距离在0.000 ~ 0.032之间，序列可划分为14个单倍型，Hd和Pi分别为0.604和0.23×10-2。对序列进行了结构分析。结果表明，该matK序列可用于茶树大田基因库DNA条形码的开发和利用。

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Analysis of DNA Barcoding Suitable for Tea Tree Field Genebank

With the development of research for DNA barcoding, its application has attracted more and more attention. In this study, 100 tea tree samples were selected as subjects, the partial sequences of chloroplast matK and rbcL genes were used to investigate the molecular barcodes suitable for tea tree The results showed that the rbcL sequences of 100 tea samples were identical, the matK sequences were different, the genetic distance ranged from 0.000 to 0.032, the sequences could be divided into 14 haplotypes, Hd and Pi were 0.604 and 0.23×10-2, at the same time, the construction analysis of the sequence is carried out. The results showed that the matK sequence could be used in the development and utilization of DNA barcoding of tea tree field genebank.

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来源期刊

茶叶科学

CiteScore

1.50

自引率

0.00%

发文量

2558

期刊介绍： The Journal of Tea Science was established in August 1964, approved by the Publicity Department, CCCPC. Its title was inscribed by Zhu De, the chairman of CCCPC. It was discontinued during the Cultural Revolution in 1966, and it was reissued in August 1984, approved by the State Scientific and Technological Commission.Academicians Chen Zongmao and Liu Zhonghuaof the Chinese Academy of Engineering served as the directors of the editorial board. The Journal of Tea Science is managed by the China Association for Science and Technology,sponsored by the China Tea Science Society and the Tea Research Institute of the Chinese Academy of Agricultural Sciences, and edited and published by the editorial office of the Journal of Tea Science. It is the only one of Chinese core journals in the field of tea science that is included in the core library of the Chinese Science Citation Database.Its Domestic Unified Serial Number is CN 33-1115/S, its International Standard Serial Number is ISSN 1000-369X and its International publication name code is CODEN-CHKEF4. At present, the Journal of Tea Science is a bimonthly publication, published in the middle of the month, with a book size of 16.