胡萝卜籽溶剂组分对血红蛋白糖基化、葡萄糖摄取及体外抗糖尿病活性的抑制作用

H. Tijjani, S. A. Imam
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引用次数: 4

摘要

糖尿病(DM)是一种由绝对或相对胰岛素缺乏引起的代谢性疾病,可导致某些器官系统功能障碍。抑制胰α-葡萄糖苷酶和α-淀粉酶是通过淀粉控制分解降低餐后高血糖水平的有效策略。该研究的目的是评估胡萝卜籽提取物的体外抗糖尿病活性,胡萝卜籽提取物是一种传统上用于治疗糖尿病的植物。用乙酸乙酯(EAE)、正己烷(HEX)和乙醚(DEE)对胡萝卜籽水提物(AQE)进行分割,得到不同馏分。AQE和EAE具有显著的α -淀粉酶抑制活性,IC50值分别为637.0±18.6 μg/mL和603.0±25.8 μg/mL。AQE、EAE、HEX和DEE表达α -葡萄糖苷酶抑制活性,IC50值分别为135.85±1.21、147.59±0.57、132.64±1.17和143.56±0.49 μg/mL,而阿卡波糖(ACA)的IC50值为5.42±0.20 μg/mL。此外,DEE组分对酵母细胞%葡萄糖摄取的抑制作用与甲硝唑相当。在200 ~ 1000 μg/mL浓度下,胡萝卜提取物对血红蛋白糖基化有不同程度的抑制作用。结果表明,胡萝卜籽水提物具有体外抗糖尿病作用,其中EAE和HEX对α-淀粉酶和α-葡萄糖苷酶的抑制作用最强。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Inhibition of Haemoglobin Glycosylation, Glucose Uptake and In Vitro Antidiabetic Activities of Solvent Fractions from Daucus carota Seed
Abstract Diabetes mellitus (DM) is a metabolic disorder which result from either absolute or relative insulin deficiency and may lead to dysfunction in some organ systems. Pancreatic α- glucosidase and α- amylase inhibition are an effective strategy to decrease levels of postprandial hyperglycemia through starch control breakdown. The aim of the study is to assess the in vitro antidiabetic activities of fractions from Daucus carota seed extract, a plant used traditionally for the treatment of diabetes mellitus. The aqueous extract (AQE) of Daucus carota seed was partitioned in ethyl acetate (EAE), n – hexane (HEX) and diethyl ether (DEE) to obtain the various fractions. The AQE and EAE expressed significant α – amylase inhibitory activity with IC50 values of 637.0±18.6 μg/mL and 603.0±25.8 μg/mL respectively. AQE, EAE, HEX and DEE expressed α – glucosidase inhibitory activity with IC50 value of 135.85±1.21, 147.59±0.57, 132.64±1.17, and 143.56±0.49 μg/mL respective compared with acarbose (ACA) with 5.42±0.20 μg/mL. Furthermore, DEE fraction expressed inhibitory effects on % glucose uptake in yeast cell comparable with metronidazole. All Daucus carota fractions expressed various inhibitory effects on haemoglobin glycosylation at a concentration of 200 – 1000 μg/mL. The results show that fractions from the aqueous seed extract of Daucus carota possess in vitro antidiabetic potentials with EAE and HEX fractions having most promising inhibitory activities against α-amylase and α-glucosidase respectively.
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