近场单分子光谱

Sunney Xie, P. Lu, R. Bian, E. Sanchez, P. Leung
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摘要

近场扫描光学显微镜(NSOM)的最新进展使单分子发射成像具有纳米分辨率,并将在低温下完成的单分子光谱工作扩展到室温状态。有了NSOM,分子荧光可以在光谱和时间上进行分辨,允许在单分子基础上以超过衍射极限的空间分辨率详细研究分子相互作用和动力学。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Near-field single molecule spectroscopy
Recent advances in near-field scanning optical microscopy (NSOM) make it possible to image single molecule emission with nanometric resolution and extend the single molecule spectroscopy work done at cryogenic temperature to the room temperature regime. With NSOM, molecular fluorescence can be spectrally and temporally resolved, allowing detailed studies of molecular interaction and dynamics on a single molecule basis with a spatial resolution beyond the diffraction limit.
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