{"title":"用于遗传毒性和毒性生物测定的简单两步沉积物提取程序","authors":"K. Kwan, B. Dutka","doi":"10.1002/TOX.2540050407","DOIUrl":null,"url":null,"abstract":"Three simple sediment extraction procedures, using Milli-Q water, DMSO, and methanol, were applied to 16 sediment samples collected from the Thames River, Ontario, Canada. The three extracts were tested for the presence of toxicity and genotoxicity using the Microtox,® Toxi-Chromotest,® and Mutatox® (with and without S9) procedures. Results of the study indicated that the Mutatox genotoxicity screening test was an extremely sensitive procedure responding to chemicals in all three types of extracts. Methanol was found to be more efficient than DMSO in extracting toxic and genotoxic chemicals from these sediments. Procedural details and results are discussed.","PeriodicalId":11824,"journal":{"name":"Environmental Toxicology & Water Quality","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1990-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"55","resultStr":"{\"title\":\"Simple two‐step sediment extraction procedure for use in genotoxicity and toxicity bioassays\",\"authors\":\"K. Kwan, B. Dutka\",\"doi\":\"10.1002/TOX.2540050407\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Three simple sediment extraction procedures, using Milli-Q water, DMSO, and methanol, were applied to 16 sediment samples collected from the Thames River, Ontario, Canada. The three extracts were tested for the presence of toxicity and genotoxicity using the Microtox,® Toxi-Chromotest,® and Mutatox® (with and without S9) procedures. Results of the study indicated that the Mutatox genotoxicity screening test was an extremely sensitive procedure responding to chemicals in all three types of extracts. Methanol was found to be more efficient than DMSO in extracting toxic and genotoxic chemicals from these sediments. Procedural details and results are discussed.\",\"PeriodicalId\":11824,\"journal\":{\"name\":\"Environmental Toxicology & Water Quality\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1990-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"55\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Environmental Toxicology & Water Quality\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1002/TOX.2540050407\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental Toxicology & Water Quality","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/TOX.2540050407","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Simple two‐step sediment extraction procedure for use in genotoxicity and toxicity bioassays
Three simple sediment extraction procedures, using Milli-Q water, DMSO, and methanol, were applied to 16 sediment samples collected from the Thames River, Ontario, Canada. The three extracts were tested for the presence of toxicity and genotoxicity using the Microtox,® Toxi-Chromotest,® and Mutatox® (with and without S9) procedures. Results of the study indicated that the Mutatox genotoxicity screening test was an extremely sensitive procedure responding to chemicals in all three types of extracts. Methanol was found to be more efficient than DMSO in extracting toxic and genotoxic chemicals from these sediments. Procedural details and results are discussed.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
