摘要铜绿假单胞菌opf蛋白和aTox蛋白免疫小鼠白细胞,增强白细胞的吞噬和杀菌活性

Q3 Medicine
E. Kalinichenko, S. Skhodova, N. Akhmatova, N. Mikhailova
{"title":"摘要铜绿假单胞菌opf蛋白和aTox蛋白免疫小鼠白细胞,增强白细胞的吞噬和杀菌活性","authors":"E. Kalinichenko, S. Skhodova, N. Akhmatova, N. Mikhailova","doi":"10.36233/0372-9311-2018-2-10-15","DOIUrl":null,"url":null,"abstract":"Aim. To study the effect of vaccine preparation against Pseudomonas aeruginosa on the phagocytic and bactericidal activity of immunocompetent cells in mice. Materials and methods. Preparation: 25 pg of OprF, 50 pg of aTox sorbed by 75 pg of aluminum hydroxide. For immunization, the recombinant protein preparation was mixed in equal weight fractions with an aluminum hydroxide gel, diluted in phosphate buffered saline, and sorbed for 12 hours at 4°C. The vaccine preparation was administered intraperitoneally in 0.5 ml to BALB/с mice. The phagocytic activity of peripheral blood leukocytes was determined from the absorption capacity of heat-killed FITC-labeled Staphylococcus aureus by neutrophils and monocytes of immunized mice by flow cytometry. The bactericidal activity of mice blood leukocytes was assessed for the live culture of S.aureus using flow cytometry Cytomix FC-500 (Beckman Coulter). Results. Administration to mice of recombinant proteins P. aeruginosa OprF and aTox sorbed on aluminum hydroxide led to an increase in the phagocytic and bactericidal activity of monocytes and granulocytes of peripheral blood. The maximum increase in the number of phagocytized monocytes was observed on the 7th, and granulocytes on the 17th day after the first immunization. Booster immunization did not lead to additional stimulation of phagocytic activity, but the number of phagocytic cells was significantly (p<0.05) higher than control (intact mice). Conclusion. Candidate vaccine against P. aeruginosa based on its recombinant proteins OprF and aTox activates the cellular unit of the immune system with the induction of the activity of professional macrophages.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"201 1","pages":"10-15"},"PeriodicalIF":0.0000,"publicationDate":"2018-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"IMMUNIZATION WITH PROTEINS OF PSEUDOMONAS AERUGINOSA OprF AND aTox ENHANCES THE PHAGOCYTIC AND BACTERICIDAL ACTIVITY OF LEUKOCYTES IN MICE\",\"authors\":\"E. Kalinichenko, S. Skhodova, N. Akhmatova, N. Mikhailova\",\"doi\":\"10.36233/0372-9311-2018-2-10-15\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Aim. To study the effect of vaccine preparation against Pseudomonas aeruginosa on the phagocytic and bactericidal activity of immunocompetent cells in mice. Materials and methods. Preparation: 25 pg of OprF, 50 pg of aTox sorbed by 75 pg of aluminum hydroxide. For immunization, the recombinant protein preparation was mixed in equal weight fractions with an aluminum hydroxide gel, diluted in phosphate buffered saline, and sorbed for 12 hours at 4°C. The vaccine preparation was administered intraperitoneally in 0.5 ml to BALB/с mice. The phagocytic activity of peripheral blood leukocytes was determined from the absorption capacity of heat-killed FITC-labeled Staphylococcus aureus by neutrophils and monocytes of immunized mice by flow cytometry. The bactericidal activity of mice blood leukocytes was assessed for the live culture of S.aureus using flow cytometry Cytomix FC-500 (Beckman Coulter). Results. Administration to mice of recombinant proteins P. aeruginosa OprF and aTox sorbed on aluminum hydroxide led to an increase in the phagocytic and bactericidal activity of monocytes and granulocytes of peripheral blood. The maximum increase in the number of phagocytized monocytes was observed on the 7th, and granulocytes on the 17th day after the first immunization. Booster immunization did not lead to additional stimulation of phagocytic activity, but the number of phagocytic cells was significantly (p<0.05) higher than control (intact mice). Conclusion. Candidate vaccine against P. aeruginosa based on its recombinant proteins OprF and aTox activates the cellular unit of the immune system with the induction of the activity of professional macrophages.\",\"PeriodicalId\":24020,\"journal\":{\"name\":\"Zhurnal mikrobiologii, epidemiologii, i immunobiologii\",\"volume\":\"201 1\",\"pages\":\"10-15\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2018-04-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zhurnal mikrobiologii, epidemiologii, i immunobiologii\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.36233/0372-9311-2018-2-10-15\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.36233/0372-9311-2018-2-10-15","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 0

摘要

的目标。目的研究铜绿假单胞菌疫苗制备对小鼠免疫活性细胞吞噬和杀菌活性的影响。材料和方法。制备:25pg OprF, 50pg aTox,用75pg氢氧化铝吸附。免疫接种时,将重组蛋白制剂与氢氧化铝凝胶等量混合,用磷酸盐缓冲盐水稀释,在4℃下吸附12小时。将疫苗制剂0.5 ml灌胃BALB/小鼠。通过流式细胞术检测免疫小鼠的中性粒细胞和单核细胞对热杀伤fitc标记金黄色葡萄球菌的吸收能力,测定外周血白细胞的吞噬活性。采用Beckman Coulter公司流式细胞仪(Cytomix FC-500)对金黄色葡萄球菌活培养小鼠血液白细胞的杀菌活性进行了评价。结果。将氢氧化铝吸附的重组蛋白P. aeruginosa OprF和aTox注入小鼠,可提高小鼠外周血单核细胞和粒细胞的吞噬和杀菌活性。第一次免疫后第7天吞噬单核细胞数量增加最多,第17天吞噬粒细胞数量增加最多。强化免疫未引起吞噬活性的额外刺激,但吞噬细胞数量显著高于对照组(完整小鼠)(p<0.05)。结论。基于重组蛋白OprF和aTox的铜绿假单胞菌候选疫苗通过诱导专业巨噬细胞的活性激活免疫系统的细胞单位。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
IMMUNIZATION WITH PROTEINS OF PSEUDOMONAS AERUGINOSA OprF AND aTox ENHANCES THE PHAGOCYTIC AND BACTERICIDAL ACTIVITY OF LEUKOCYTES IN MICE
Aim. To study the effect of vaccine preparation against Pseudomonas aeruginosa on the phagocytic and bactericidal activity of immunocompetent cells in mice. Materials and methods. Preparation: 25 pg of OprF, 50 pg of aTox sorbed by 75 pg of aluminum hydroxide. For immunization, the recombinant protein preparation was mixed in equal weight fractions with an aluminum hydroxide gel, diluted in phosphate buffered saline, and sorbed for 12 hours at 4°C. The vaccine preparation was administered intraperitoneally in 0.5 ml to BALB/с mice. The phagocytic activity of peripheral blood leukocytes was determined from the absorption capacity of heat-killed FITC-labeled Staphylococcus aureus by neutrophils and monocytes of immunized mice by flow cytometry. The bactericidal activity of mice blood leukocytes was assessed for the live culture of S.aureus using flow cytometry Cytomix FC-500 (Beckman Coulter). Results. Administration to mice of recombinant proteins P. aeruginosa OprF and aTox sorbed on aluminum hydroxide led to an increase in the phagocytic and bactericidal activity of monocytes and granulocytes of peripheral blood. The maximum increase in the number of phagocytized monocytes was observed on the 7th, and granulocytes on the 17th day after the first immunization. Booster immunization did not lead to additional stimulation of phagocytic activity, but the number of phagocytic cells was significantly (p<0.05) higher than control (intact mice). Conclusion. Candidate vaccine against P. aeruginosa based on its recombinant proteins OprF and aTox activates the cellular unit of the immune system with the induction of the activity of professional macrophages.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Zhurnal mikrobiologii, epidemiologii, i immunobiologii
Zhurnal mikrobiologii, epidemiologii, i immunobiologii Immunology and Microbiology-Immunology and Microbiology (miscellaneous)
CiteScore
1.40
自引率
0.00%
发文量
51
审稿时长
8 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信