Lecitase®ultra在可回收聚合物载体上的固定化:在有机溶剂中反式甲基(4-甲氧基苯基)甘氨酸酯溶解中的应用

Q2 Chemistry

Tetrahedron, asymmetry Pub Date : 2017-11-15 DOI:10.1016/j.tetasy.2017.10.006

Thenkrishnan Kumaraguru, Tirunagari Harini, Shalini Basetty

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引用次数: 11

摘要

通过吸附和戊二醛交联，将leitase®Ultra固定在聚乙烯亚胺功能化的环氧活化聚合物(DILBEAD-VWR)上。在二甲苯中进行反式-(±)-3-(4-甲氧基苯基)甘氨酸甲酯的分离(即99%，转化率50%)。该酶不受水解过程中产生的4-甲氧基苯乙醛的抑制，并且固定化酶在7%的水分含量下在有机相中有效地工作。固定化酶可以多次回收，用1 M HCl洗涤去除固定化酶后，聚合物载体也可以回收。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Immobilization of Lecitase® ultra on recyclable polymer support: application in resolution of trans-methyl (4-methoxyphenyl)glycidate in organic solvents

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Immobilization of Lecitase® ultra on recyclable polymer support: application in resolution of trans-methyl (4-methoxyphenyl)glycidate in organic solvents

Lecitase® Ultra was immobilized on epoxy-activated polymer (DILBEAD-VWR) functionalized with polyethyleneimine via adsorption and crosslinking with glutaraldehyde. The resolution of methyl trans-(±)-3-(4-methoxyphenyl) glycidate was carried out in xylene (e.e. >99%, conversion 50%). The enzyme is not inhibited by the 4-methoxy phenyl acetaldehyde produced during hydrolysis and the immobilized enzyme with 7% moisture content works efficiently in an organic phase. While the immobilized enzyme can be recycled several times, the polymer support can also be recycled after removing the immobilized enzyme by washing with 1 M HCl.

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来源期刊

Tetrahedron, asymmetry 化学-无机化学与核化学

CiteScore

4.70

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： Cessation. Tetrahedron: Asymmetry presents experimental or theoretical research results of outstanding significance and timeliness on asymmetry in organic, inorganic, organometallic and physical chemistry, as well as its application to related disciplines, especially bio-organic chemistry. The journal publishes critical reviews, original research articles and preliminary communications dealing with all aspects of the chemical, physical and theoretical properties of non-racemic organic and inorganic materials and processes. Topics relevant to the journal include: the physico-chemical and biological properties of enantiomers; strategies and methodologies of asymmetric synthesis; resolution; chirality recognition and enhancement; analytical techniques for assessing enantiomeric purity and the unambiguous determination of absolute configuration; and molecular graphics and modelling methods for interpreting and predicting asymmetric phenomena. Papers describing the synthesis or properties of non-racemic molecules will be required to include a separate statement in the form of a Stereochemistry Abstract, for publication in the same issue, of the criteria used for the assignment of configuration and enantiomeric purity.