根状农杆菌转化六足鬼臼培养物提高鬼臼毒素产量的研究

Natural Product Letters Pub Date : 2001-10-01 DOI:10.1080/10575630108041286

A. Giri, C. C. Giri, V. Dhingra, M. Narasu

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引用次数: 46

摘要

摘要鬼臼毒素是一种强效的化疗药物，是从鬼臼中提取的。利用不同的发根农杆菌菌株A4、15834、K599转化了六心豆胚。确定愈伤组织的转化性质，并进一步将培养维持为单克隆。转化培养物的HPLC分析显示，与对照相比，鬼臼毒素含量增加了三倍。

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Enhanced Podophyllotoxin Production from Agrobacterium Rhizogenes Transformed Cultures of Podophyllum Hexandrum

Abstract Podophyllotoxin, a potent chemotherapeutic agent is obtained from Podophylhum hexandrum Royle. Embryos of P. hexandrum were transformed using different strains of Agrobacterium rhizogenes viz. A4, 15834, K599. Transformed nature of the calli was ascertained and the cultures were further maintained as individual clones. HPLC analysis of transformed cultures depicted a three-fold increase in podophyllotoxin content in comparison to controls.

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Natural Product Letters

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