切除青蛙心脏中的肥胖抑制素信号

IF 0.3 4区综合性期刊 Q4 MULTIDISCIPLINARY SCIENCES

Comptes Rendus De L Academie Bulgare Des Sciences Pub Date : 2013-01-01 DOI:10.7546/CR-2013-66-6-13101331-10

Iliyana Sazdova, Bilyana M. Ilieva, M. Shkodrova, A. Milusheva, M. Chichova, R. Schubert, Ferenc Fülüp, L. Lubomirov, H. Gagov

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引用次数: 0

摘要

本研究的目的是利用溶解于或添加于0.1%或0.01%二甲亚砜(DMSO)的药理学工具，研究肥胖抑制素在体外心脏制剂中的信号传导作用。在浓度范围1-1000 nmol/l范围内，增加肥胖抑制素的用量显著降低了0.1% DMSO存在下切除青蛙心脏的收缩力。心得安在DMSO存在的情况下完全抑制了肥胖抑制素的作用，而不影响收缩力的幅度。百日咳毒素、PP2和U0126完全阻断了肥胖诱导的青蛙心脏收缩力的下降。在XE991存在的情况下，10 mol/l的肥胖抑素对相同制剂的最大收缩力没有影响。当与camp依赖性蛋白激酶(PKA)抑制剂一起应用时，Obestatin降低了收缩力。这表明，在0.1% DMSO存在下，肥胖抑制素的负性肌力效应是由于神经元KCNQ通道调节，随后是-肾上腺素能受体依赖和pka独立的收缩力下降。我们的数据首次揭示了神经元MAPK激酶参与心脏中的肥胖抑制素信号传导。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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OBESTATIN SIGNALLING IN EXCISED FROG HEART

The aim of this research is to study the obestatin signalling on in vitro heart preparations of Rana ridibunda frog using pharmacological tools dissolved in or added to 0.1% or 0.01% dimethyl sulfoxide (DMSO). The application of increasing amounts of obestatin in the concentration range 1–1000 nmol/l significantly decreased the force of contraction of excised frog hearts in the presence of 0.1% DMSO. Propranolol entirely inhibited the effect of obestatin in the presence of DMSO without affecting the amplitudes of the force of contraction. Pertussis toxin, PP2 and U0126 completely blocked the obestatininduced decrease of the force of frog heart contractions. In the presence of XE991, 10 mol/l obestatin had no effect on the maximal force of contraction of the same preparations. Obestatin decreased the force of contraction when applied together with an inhibitor of cAMP-dependent protein kinase (PKA). It is suggested that the negative inotropic effect of obestatin observed in the presence of 0.1% DMSO is due to neuronal KCNQ channels modulation followed by a �-adrenergic receptor-dependent and PKA-independent decrease of the contraction force. Our data reveal for the first time the participation of neuronal MAPK kinase in obestatin signalling in the heart.

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来源期刊

Comptes Rendus De L Academie Bulgare Des Sciences 综合性期刊-综合性期刊

CiteScore

0.60

自引率

33.30%

发文量

181

审稿时长

3-6 weeks

期刊介绍： Founded in 1948 by academician Georgy Nadjakov, "Comptes rendus de l’Académie bulgare des Sciences" is also known as "Доклади на БАН","Доклады Болгарской академии наук" and "Proceeding of the Bulgarian Academy of Sciences". If applicable, the name of the journal should be abbreviated as follows: C. R. Acad. Bulg. Sci. (according to ISO)