热带水生和土壤环境中细菌对单宁酸的利用

E. Olumuyiwa, O. Amund, M. Ilori, S. Schmidt
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引用次数: 1

摘要

从拉各斯泻湖水和红树林土壤样品中分离到2株菌株EO1和EO2。根据形态学和生理特征以及16S rRNA基因序列分析,鉴定为肺炎克雷伯菌EO1和塞浦路斯Pantoea菌株EO2,加入号分别为KX355800和KX355801。在好氧间歇条件下进行了三次微生物生长评估。这两株菌株能够利用单宁酸、没食子酸、葡萄糖、琥珀酸、苯甲酸和原儿茶酸作为唯一的碳源。生长实验和酶学研究表明,微生物通过没食子酸和邻苯三酚作为代谢中间体水解单宁酸。这些生物具有消除尼日利亚河口环境中与木材残留物有关的没食子丹宁等多酚类污染物的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
UTILIZATION OF TANNIC ACID BY BACTERIA IN TROPICAL AQUATIC AND SOIL ENVIRONMENTS
Two bacterial strains designated as EO1 and EO2, were isolated by selective culture enrichment from Lagos lagoon water and mangrove soil samples. They were subsequently identified based on morphological and physiological characteristics as well as 16S rRNA gene sequence analysis as Klebsiella pneumoniae strain EO1 and Pantoea cypripedii strain EO2 with accession numbers KX355800 and KX355801. Microbial growth assessment was performed in triplicates under aerobic batch conditions. The two isolates were capable of utilizing tannic acid, gallic acid, glucose, succinate, benzoic acid, and protocatechuic acid as sole carbon sources. Growth experiments along with enzymatic studies indicated that the organisms hydrolyzed tannic acid through gallic acid and pyrogallol as metabolic intermediates. The organisms have potential for the elimination of polyphenolic pollutants such as gallotannins associated with wood residues in Nigerian estuarine environments.
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