{"title":"从血液中分离淋巴细胞。将尼龙纤维过滤和差动离心相结合的程序。","authors":"A. Tärnvik","doi":"10.1111/J.1699-0463.1970.TB04308.X","DOIUrl":null,"url":null,"abstract":"Lymphocytes were isolated from human blood by a simple procedure combining nylon fibre filtration and centrifugation in colloidal silica-polyvinylpyrrolidone. In the preparation obtained 99.5 ± 0.6 per cent of the leukocytes were lymphocytes; it contained 0–202, mostly less than 20, platelets, and 0–1.5, mostly 0, RBC per 100 leukocytes. The lymphocyte recovery was 13.3 ± 3.3 per cent. In the trypan blue test 2.2 ± 3.0 per cent of the cells were stained.","PeriodicalId":7323,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology","volume":"115 1","pages":"311-6"},"PeriodicalIF":0.0000,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"8","resultStr":"{\"title\":\"Isolation of lymphocytes from blood. A procedure combining nylon fibre filtration and differential centrifugation.\",\"authors\":\"A. Tärnvik\",\"doi\":\"10.1111/J.1699-0463.1970.TB04308.X\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Lymphocytes were isolated from human blood by a simple procedure combining nylon fibre filtration and centrifugation in colloidal silica-polyvinylpyrrolidone. In the preparation obtained 99.5 ± 0.6 per cent of the leukocytes were lymphocytes; it contained 0–202, mostly less than 20, platelets, and 0–1.5, mostly 0, RBC per 100 leukocytes. The lymphocyte recovery was 13.3 ± 3.3 per cent. In the trypan blue test 2.2 ± 3.0 per cent of the cells were stained.\",\"PeriodicalId\":7323,\"journal\":{\"name\":\"Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology\",\"volume\":\"115 1\",\"pages\":\"311-6\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2009-08-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"8\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1111/J.1699-0463.1970.TB04308.X\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1111/J.1699-0463.1970.TB04308.X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Isolation of lymphocytes from blood. A procedure combining nylon fibre filtration and differential centrifugation.
Lymphocytes were isolated from human blood by a simple procedure combining nylon fibre filtration and centrifugation in colloidal silica-polyvinylpyrrolidone. In the preparation obtained 99.5 ± 0.6 per cent of the leukocytes were lymphocytes; it contained 0–202, mostly less than 20, platelets, and 0–1.5, mostly 0, RBC per 100 leukocytes. The lymphocyte recovery was 13.3 ± 3.3 per cent. In the trypan blue test 2.2 ± 3.0 per cent of the cells were stained.
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