中蟹提取物对黑色素合成的抑制作用

Q4 Pharmacology, Toxicology and Pharmaceutics

Korean Journal of Pharmacognosy Pub Date : 2020-01-01 DOI:10.22889/KJP.2020.51.3.171

Leejin Lim, Byeongsam Chu, S. Lee, Bounleuane Douangdeuane, Heesang Song

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引用次数: 0

摘要

摘要/ abstract摘要:本研究目的是研究Decaschistia intermedia crab (EDI)提取物的抗黑素生成作用。在这项研究中，我们检测了EDI对体外蘑菇酪氨酸酶活性、黑色素含量以及B16F10黑色素瘤细胞中黑色素生成相关基因mRNA和蛋白表达水平的影响。EDI处理显著降低B16F10细胞酪氨酸酶活性和黑色素含量，且呈剂量依赖性。此外，我们发现，与对照组相比，在edii处理的B16F10细胞中，a-黑色素细胞刺激激素(aMSH)诱导的小眼相关转录因子(MITF)、酪氨酸酶、酪氨酸酶相关蛋白-1 (TRP-1)和TRP-2等黑色素生成蛋白的mRNA或蛋白的表达呈剂量依赖性显著下调。我们的研究结果表明，EDI通过下调a- msh刺激的黑色素合成来抑制细胞黑色素生成。因此EDI可能是一种有效的增白剂。

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Inhibitory Effects of Decaschistia intermedia Craib Extract on Melanin Synthesis

 The purpose of this study was to investigate the anti-melanogenic effects of the extracts from Decaschistia intermedia craib (EDI). In this study, we examined the effects of EDI on mushroom tyrosinase activity in in vitro, melanin contents, and expression levels of mRNA and proteins of melanogenesis-related genes in B16F10 melanoma cells. The treatment of EDI significantly decreased both tyrosinase activity and melanin contents in B16F10 cells with dose-dependent manner. In addition, we found that the expression of mRNA or proteins of melanogenic proteins, such as, a-melanocyte-stimulating hormone (aMSH)-induced microphthalmia associated transcription factor (MITF), tyrosinase, tyrosinase related protein-1 (TRP-1), and TRP-2 was significantly downregulated with dose-dependent manner in the EDI-treated B16F10 cells compared to controls. Our results suggest that the EDI inhibits cellular melanogenesis through downregulation of a-MSH-stimulated melanin synthesis. Thus EDI may potentially be an effective whitening agent.

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来源期刊

Korean Journal of Pharmacognosy Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science

CiteScore

0.70

自引率

0.00%

发文量