儿童哮喘与 Gasdermin B (GSDMB) 和 Oromucoid-like 3 (ORMDL3) 基因的关系。

IF 0.9 Q3 MEDICINE, GENERAL & INTERNAL
Northern Clinics of Istanbul Pub Date : 2023-11-28 eCollection Date: 2023-01-01 DOI:10.14744/nci.2023.22120
Mehmet Almacioglu, Ozlem Keskin, Mehmet Yasar Ozkars, Sibel Oguzkan Balci, Ercan Kucukosmanoglu, Sacide Pehlivan, Mehmet Keskin
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引用次数: 0

摘要

研究目的基因组长度关联研究表明,位于第17号染色体长臂上的Gasdermin B(GSDMB)和Orosomucoid-like 3(ORMDL3)基因与哮喘有关。本研究旨在确定哮喘控制试验(ACT)、运动激发试验(ECT)和一氧化氮分数(FENO)水平与 GSDMB 和 ORMDL3 基因表达之间可能存在的关系。我们将患者分为两个亚组,即轻度持续性哮喘(29 名)和中度持续性哮喘(30 名)。对所有病例的 ORMDL3、GSDMB 基因表达水平、ECT、总 IgE 水平和嗜酸性粒细胞计数进行了测量。此外,还测量了哮喘患儿的 ACT 和 FeNO 水平。随后,研究了ORMDL3和GSDMB基因表达系数的变化与ECT、ACT和FeNO的关系:结果:ACT≤15的患者与ACT≥20的患者相比,ORMDL3和GSDMB基因表达量分别增加了6.74倍和11.74倍。比较 ACT ≥20 和 ACT ≤15 患者的系数变化(ΔCq),观察到 ACT ≤15 患者 ORMDL3 的 ΔCq 变化值更高(p=0.015)。同样,当 FENO≤25 ppb 的患者与 FENO >25 ppb 的患者相比,ORMDL3 和 GSDMB 基因表达量分别增加了 2.93 倍和 3.56 倍。在比较系数变化时,FENO≤25 和 FENO >25 患者之间没有发现明显差异。ΔCq值与ACT评分之间存在轻微的负相关(ORMDL3为p=0.003,r=-0.418;GSDMB为p=0.016,r=-0.345)。此外,我们还观察到 ORMDL3 和 GSDMB 基因表达之间存在统计学意义上的显著正相关(r=0.80,pConclusion):我们的研究表明,ORMDL3 和 GSDMB 基因表达水平的升高可能与哮喘患者的 ACT 评分、FeNO 和 ECT 相关。这些发现可能会鼓励未来开展更多受试者的研究,从而利用各种哮喘表型的基因表达水平进行预后预测。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Association of childhood asthma with Gasdermin B (GSDMB) and Oromucoid-like 3 (ORMDL3) genes.

Objective: Genome-length association studies have shown that Gasdermin B (GSDMB) and Orosomucoid-like 3 (ORMDL3) genes located on the long arm of chromosome 17 are associated with asthma. In this study, it was aimed to determine the possible relationship between asthma control test (ACT), exercise provocation test (ECT), and fractional nitric oxide (FENO) levels and GSDMB and ORMDL3 gene expressions.

Methods: 59 asthmatic and 38 non-asthmatic children were included in the study. We divided the patient group into two subgroups as mild persistent asthma (29 patients) and moderate persistent asthma (30 patients). ORMDL3, GSDMB gene expression levels, ECT, total IgE levels, and eosinophil counts were measured in all cases. In addition, ACT and FeNO levels were measured in children with asthma. Afterward, the relationship of ORMDL3 and GSDMB gene expression coefficient changes with ECT, ACT, and FeNO was examined.

Results: When patients with ACT ≤15 were compared with patients with ACT ≥20, ORMDL3 and GSDMB gene expressions were increased 6.74 and 11.74 times, respectively. Comparing patients with ACT ≥20 and ACT ≤15 in terms of coefficient changes (ΔCq), higher change values were observed for ΔCq ORMDL3 in patients with ACT ≤15 (p=0.015). Similarly, when patients with FENO ≤25 ppb were compared with patients with FENO >25 ppb, ORMDL3 and GSDMB gene expressions were increased by 2.93 and 3.56 times, respectively. When the coefficient changes were compared, no significant difference was found between FENO≤25 and FENO >25 patients. There was a slight negative correlation between ΔCq values and ACT score (p=0.003, r=-0.418 for ORMDL3, and p=0.016, r=-0.345 for GSDMB). In addition, we observed a statistically significant positive correlation between ORMDL3 and GSDMB gene expressions (r=0.80, p<0.001).

Conclusion: We showed that increased ORMDL3 and GSDMB gene expression levels may be associated with ACT scores, FeNO and ECT in asthma. These findings may encourage future studies with larger numbers of subjects that can use gene expression levels in various asthma phenotypes for prognostic prediction.

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Northern Clinics of Istanbul
Northern Clinics of Istanbul MEDICINE, GENERAL & INTERNAL-
CiteScore
0.40
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0.00%
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48
审稿时长
10 weeks
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