[猪和仔猪冷冻心脏碎片提取物的肽组成]。

L. A. Rohoza, T. S. Diubko, S. I. Hal'chenko, B. P. Sandomyrs'kyĭ
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引用次数: 0

摘要

众所周知,冷冻保存的猪和仔猪器官碎片提取物可刺激修复性再生过程。因此,对其提取物的研究对了解其生物学作用机制具有重要意义。本文研究了猪和仔猪冷冻心脏片段提取物中多肽的分子质量分布,即猪心脏提取物的色谱为3个峰,仔猪心脏提取物的色谱为6个峰。猪心脏提取物的特点是缺乏分子质量为10,000或更多的肽。提取液荧光强度的差异说明猪心脏提取液中含有较多的可溶色氨酸残基。获得了提取物的同步荧光光谱,可以在不评估其成分的情况下对提取物进行鉴定。本研究结果可用于心脏提取物多肽组成的控制和标准化,并对其生物品质进行研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Peptide composition of extracts of cryopreserved pigs' and piglets' heart fragments].
It is known that extracts of cryopreserved organ fragments of pigs and piglets stimulate the processes of reparative regeneration. Therefore, the study of the extracts is essential for understanding the mechanism of their biological effects. In this paper was studied the molecular mass distribution of peptides in the extracts of cryopreserved heart fragments of pigs' and piglets', i.e., the chromatograms of pigs' heart extracts show 3 picks, whilst piglets' heart extract show 6 picks. The peculiarity of pigs' heart extracts is the absence of peptides with molecular mass of 10,000 and more. The differences in the intensity of extracts fluorescence prove that the peptides in pigs' heart extracts contain greater amount of tryptophan residues accessible for solvent. The synchronous fluorescence spectra of extracts were obtained which allows the identification of extract without assessment of their components. Results shown in this research could be used for control and standardization of heart extracts peptide composition under investigation of their biological quality.
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