{"title":"豌豆系统感染红三叶草斑驳病毒后蛋白质形态的变化","authors":"Kamil Marcinka , Cornelia Röhring , Siegfried Kluge","doi":"10.1016/S0015-3796(11)80006-5","DOIUrl":null,"url":null,"abstract":"<div><p>Proteins of healthy pea plants (<em>Pisum sativum</em> L. cv. Nadja) and of plants infected with red clover mottle virus were examined by one- and two-dimensional electrophoresis.</p><p>Silver-stained 2-D slab gels revealed more than ten additional protein spots with markedly changed intensity in the extracts from infected plants as compared to extracts from uninfected plants. Two of these spots corresponded to virus proteins (about 38 and 21 kDa).</p></div>","PeriodicalId":8798,"journal":{"name":"Biochemie und Physiologie der Pflanzen","volume":"188 3","pages":"Pages 187-193"},"PeriodicalIF":0.0000,"publicationDate":"1992-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0015-3796(11)80006-5","citationCount":"12","resultStr":"{\"title\":\"Changes in Protein Patterns of Pea Plants Systemically Infected with Red Clover Mottle Virus\",\"authors\":\"Kamil Marcinka , Cornelia Röhring , Siegfried Kluge\",\"doi\":\"10.1016/S0015-3796(11)80006-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Proteins of healthy pea plants (<em>Pisum sativum</em> L. cv. Nadja) and of plants infected with red clover mottle virus were examined by one- and two-dimensional electrophoresis.</p><p>Silver-stained 2-D slab gels revealed more than ten additional protein spots with markedly changed intensity in the extracts from infected plants as compared to extracts from uninfected plants. Two of these spots corresponded to virus proteins (about 38 and 21 kDa).</p></div>\",\"PeriodicalId\":8798,\"journal\":{\"name\":\"Biochemie und Physiologie der Pflanzen\",\"volume\":\"188 3\",\"pages\":\"Pages 187-193\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1992-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0015-3796(11)80006-5\",\"citationCount\":\"12\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochemie und Physiologie der Pflanzen\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0015379611800065\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemie und Physiologie der Pflanzen","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0015379611800065","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 12
摘要
健康豌豆植株(Pisum sativum L. cv.)的蛋白质。采用单、双向电泳法对红三叶草斑驳病毒侵染植物进行了检测。银染二维平板凝胶显示，与未感染植物的提取物相比，感染植物的提取物中有10多个额外的蛋白质斑点，其强度显著改变。其中两个点对应于病毒蛋白(约38和21 kDa)。
Changes in Protein Patterns of Pea Plants Systemically Infected with Red Clover Mottle Virus
Proteins of healthy pea plants (Pisum sativum L. cv. Nadja) and of plants infected with red clover mottle virus were examined by one- and two-dimensional electrophoresis.
Silver-stained 2-D slab gels revealed more than ten additional protein spots with markedly changed intensity in the extracts from infected plants as compared to extracts from uninfected plants. Two of these spots corresponded to virus proteins (about 38 and 21 kDa).
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