乌苏里蝮蛇毒液纤维蛋白(基因)裂解酶的分离纯化

International Review of Biophysical Chemistry Pub Date : 2012-04-30 DOI:10.15866/IREBIC.V3I2.1551

N. K. Burlova-Vasilieva, O. Savchuk, L. Ostapchenko

{"title":"乌苏里蝮蛇毒液纤维蛋白(基因)裂解酶的分离纯化","authors":"N. K. Burlova-Vasilieva, O. Savchuk, L. Ostapchenko","doi":"10.15866/IREBIC.V3I2.1551","DOIUrl":null,"url":null,"abstract":"This paper describes the purification of fibrino(geno)lytic enzymes from Agkistrodon blomhoffii ussuriensis venom in three chromatographic stages. Two single-stranded enzymes with molecular weight of 30 and 37 kDa were separated. Both toxins were purified using affinity, hydrophobic interaction and ion-exchange chromatography and were identified as metaloproteinase and serine protease respectively","PeriodicalId":14377,"journal":{"name":"International Review of Biophysical Chemistry","volume":"99 1","pages":"20-23"},"PeriodicalIF":0.0000,"publicationDate":"2012-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Isolation and Purification of Fibrino(geno)lytic Enzymes from Agkistrodon Blomhoffi Usuriensis Venom\",\"authors\":\"N. K. Burlova-Vasilieva, O. Savchuk, L. Ostapchenko\",\"doi\":\"10.15866/IREBIC.V3I2.1551\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"This paper describes the purification of fibrino(geno)lytic enzymes from Agkistrodon blomhoffii ussuriensis venom in three chromatographic stages. Two single-stranded enzymes with molecular weight of 30 and 37 kDa were separated. Both toxins were purified using affinity, hydrophobic interaction and ion-exchange chromatography and were identified as metaloproteinase and serine protease respectively\",\"PeriodicalId\":14377,\"journal\":{\"name\":\"International Review of Biophysical Chemistry\",\"volume\":\"99 1\",\"pages\":\"20-23\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2012-04-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Review of Biophysical Chemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.15866/IREBIC.V3I2.1551\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Review of Biophysical Chemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.15866/IREBIC.V3I2.1551","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

摘要

本文介绍了从乌苏里蝮蛇毒液中分离纯化纤维蛋白(基因)裂解酶的三个色谱阶段。分离出分子量分别为30和37 kDa的单链酶。通过亲和、疏水相互作用和离子交换层析对两种毒素进行纯化，鉴定为金属蛋白酶和丝氨酸蛋白酶

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Isolation and Purification of Fibrino(geno)lytic Enzymes from Agkistrodon Blomhoffi Usuriensis Venom

This paper describes the purification of fibrino(geno)lytic enzymes from Agkistrodon blomhoffii ussuriensis venom in three chromatographic stages. Two single-stranded enzymes with molecular weight of 30 and 37 kDa were separated. Both toxins were purified using affinity, hydrophobic interaction and ion-exchange chromatography and were identified as metaloproteinase and serine protease respectively

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

International Review of Biophysical Chemistry

自引率

0.00%

发文量