{"title":"海葛菜(亲本)在不同培养基上的半无菌培养","authors":"S. Y. Lumbessy","doi":"10.1063/1.5141331","DOIUrl":null,"url":null,"abstract":"Semi-sterile culture is an initial maintenance stage that is essential to be optimized to get sterile seaweed explants (axenic explants). The purpose of this study is to determine the effect of using culture media on the weight gainand survival rate of the Gracilaria salicornia seaweed (parental) in semi-sterile culture. This research was conducted at Tissue Culture Laboratory of Lombok Marine Aquaculture Center, Sekotong, West Nusa Tenggara. The study was carried out using three different media treatments such as Provasoli Enriched Seawater (PES), hydroponic, and Steril Sea Water (SSW) as controls. The results showed that hydroponic media have the highest average weight and survival rate of G. salicornia (48.76 g and 99.75%). The results were not significantly different from SSW media but were significantly different from PES media.Semi-sterile culture is an initial maintenance stage that is essential to be optimized to get sterile seaweed explants (axenic explants). The purpose of this study is to determine the effect of using culture media on the weight gainand survival rate of the Gracilaria salicornia seaweed (parental) in semi-sterile culture. This research was conducted at Tissue Culture Laboratory of Lombok Marine Aquaculture Center, Sekotong, West Nusa Tenggara. The study was carried out using three different media treatments such as Provasoli Enriched Seawater (PES), hydroponic, and Steril Sea Water (SSW) as controls. The results showed that hydroponic media have the highest average weight and survival rate of G. salicornia (48.76 g and 99.75%). The results were not significantly different from SSW media but were significantly different from PES media.","PeriodicalId":20577,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCE, BIOTECHNOLOGY, AND BIOMETRICS 2019","volume":"35 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Sem-sterile culture of Gracilaria salicornia seaweed (parental) on various media\",\"authors\":\"S. Y. Lumbessy\",\"doi\":\"10.1063/1.5141331\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Semi-sterile culture is an initial maintenance stage that is essential to be optimized to get sterile seaweed explants (axenic explants). The purpose of this study is to determine the effect of using culture media on the weight gainand survival rate of the Gracilaria salicornia seaweed (parental) in semi-sterile culture. This research was conducted at Tissue Culture Laboratory of Lombok Marine Aquaculture Center, Sekotong, West Nusa Tenggara. The study was carried out using three different media treatments such as Provasoli Enriched Seawater (PES), hydroponic, and Steril Sea Water (SSW) as controls. The results showed that hydroponic media have the highest average weight and survival rate of G. salicornia (48.76 g and 99.75%). The results were not significantly different from SSW media but were significantly different from PES media.Semi-sterile culture is an initial maintenance stage that is essential to be optimized to get sterile seaweed explants (axenic explants). The purpose of this study is to determine the effect of using culture media on the weight gainand survival rate of the Gracilaria salicornia seaweed (parental) in semi-sterile culture. This research was conducted at Tissue Culture Laboratory of Lombok Marine Aquaculture Center, Sekotong, West Nusa Tenggara. The study was carried out using three different media treatments such as Provasoli Enriched Seawater (PES), hydroponic, and Steril Sea Water (SSW) as controls. The results showed that hydroponic media have the highest average weight and survival rate of G. salicornia (48.76 g and 99.75%). The results were not significantly different from SSW media but were significantly different from PES media.\",\"PeriodicalId\":20577,\"journal\":{\"name\":\"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCE, BIOTECHNOLOGY, AND BIOMETRICS 2019\",\"volume\":\"35 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-12-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCE, BIOTECHNOLOGY, AND BIOMETRICS 2019\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1063/1.5141331\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCE, BIOTECHNOLOGY, AND BIOMETRICS 2019","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1063/1.5141331","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Sem-sterile culture of Gracilaria salicornia seaweed (parental) on various media
Semi-sterile culture is an initial maintenance stage that is essential to be optimized to get sterile seaweed explants (axenic explants). The purpose of this study is to determine the effect of using culture media on the weight gainand survival rate of the Gracilaria salicornia seaweed (parental) in semi-sterile culture. This research was conducted at Tissue Culture Laboratory of Lombok Marine Aquaculture Center, Sekotong, West Nusa Tenggara. The study was carried out using three different media treatments such as Provasoli Enriched Seawater (PES), hydroponic, and Steril Sea Water (SSW) as controls. The results showed that hydroponic media have the highest average weight and survival rate of G. salicornia (48.76 g and 99.75%). The results were not significantly different from SSW media but were significantly different from PES media.Semi-sterile culture is an initial maintenance stage that is essential to be optimized to get sterile seaweed explants (axenic explants). The purpose of this study is to determine the effect of using culture media on the weight gainand survival rate of the Gracilaria salicornia seaweed (parental) in semi-sterile culture. This research was conducted at Tissue Culture Laboratory of Lombok Marine Aquaculture Center, Sekotong, West Nusa Tenggara. The study was carried out using three different media treatments such as Provasoli Enriched Seawater (PES), hydroponic, and Steril Sea Water (SSW) as controls. The results showed that hydroponic media have the highest average weight and survival rate of G. salicornia (48.76 g and 99.75%). The results were not significantly different from SSW media but were significantly different from PES media.
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