{"title":"水挤压分离亚细胞组分","authors":"Renzo Rendi","doi":"10.1016/0926-6542(64)90027-7","DOIUrl":null,"url":null,"abstract":"<div><p>Suspensions of phospholipids, prepared from rat-liver mitochondria or from soya bean, extrude water when serum albumin and a Mg<sup>2+</sup>-ethylenediaminetetraacetate complex is addes. None of many other chelators tested can replace ethylenediaminetetraacetate. The pH optimum is 7.1 for the soya-bean phosphatides and 8.5 for those prepared from mitochondira. Various characteristics of the reaction are similar to the one observed in dialysed rat-liver mitochondira, although in this latter system serum albumin is not required. While in the dialyzed-mitochondira system Ca<sup>2+</sup> can be replaced by Mg<sup>2+</sup>, with the isolated phospholipid Mg<sup>2+</sup> is unable to replace Ca<sup>2</sup>. When dialyzed mitochondria are treate with proteolytic enzymes, the requirement for serum albumin is observed and one also finds that Mg<sup>2+</sup> is now unable to replace Ca<sup>2+</sup>. Little decrease of water-extrusion reaction ibn dialyzed mitochondria occurs after neutral-lipid extraction, while a large decrease is seen after phospholipid removal.</p></div>","PeriodicalId":100171,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects","volume":"84 6","pages":"Pages 694-706"},"PeriodicalIF":0.0000,"publicationDate":"1964-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6542(64)90027-7","citationCount":"14","resultStr":"{\"title\":\"Water extrusion in isolated subcellular fractions\",\"authors\":\"Renzo Rendi\",\"doi\":\"10.1016/0926-6542(64)90027-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Suspensions of phospholipids, prepared from rat-liver mitochondria or from soya bean, extrude water when serum albumin and a Mg<sup>2+</sup>-ethylenediaminetetraacetate complex is addes. None of many other chelators tested can replace ethylenediaminetetraacetate. The pH optimum is 7.1 for the soya-bean phosphatides and 8.5 for those prepared from mitochondira. Various characteristics of the reaction are similar to the one observed in dialysed rat-liver mitochondira, although in this latter system serum albumin is not required. While in the dialyzed-mitochondira system Ca<sup>2+</sup> can be replaced by Mg<sup>2+</sup>, with the isolated phospholipid Mg<sup>2+</sup> is unable to replace Ca<sup>2</sup>. When dialyzed mitochondria are treate with proteolytic enzymes, the requirement for serum albumin is observed and one also finds that Mg<sup>2+</sup> is now unable to replace Ca<sup>2+</sup>. Little decrease of water-extrusion reaction ibn dialyzed mitochondria occurs after neutral-lipid extraction, while a large decrease is seen after phospholipid removal.</p></div>\",\"PeriodicalId\":100171,\"journal\":{\"name\":\"Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects\",\"volume\":\"84 6\",\"pages\":\"Pages 694-706\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1964-12-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0926-6542(64)90027-7\",\"citationCount\":\"14\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0926654264900277\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926654264900277","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Suspensions of phospholipids, prepared from rat-liver mitochondria or from soya bean, extrude water when serum albumin and a Mg2+-ethylenediaminetetraacetate complex is addes. None of many other chelators tested can replace ethylenediaminetetraacetate. The pH optimum is 7.1 for the soya-bean phosphatides and 8.5 for those prepared from mitochondira. Various characteristics of the reaction are similar to the one observed in dialysed rat-liver mitochondira, although in this latter system serum albumin is not required. While in the dialyzed-mitochondira system Ca2+ can be replaced by Mg2+, with the isolated phospholipid Mg2+ is unable to replace Ca2. When dialyzed mitochondria are treate with proteolytic enzymes, the requirement for serum albumin is observed and one also finds that Mg2+ is now unable to replace Ca2+. Little decrease of water-extrusion reaction ibn dialyzed mitochondria occurs after neutral-lipid extraction, while a large decrease is seen after phospholipid removal.
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