不同摄食策略对蜜蜂肠道菌群及小孢子虫存在的影响

IF 0.7 4区农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE

South African Journal of Animal Science Pub Date : 2023-04-17 DOI:10.4314/sajas.v52i5.02

E. Topal, Ö. Ceylan, R. I. Tunca, V. Bay, S. Aldemir, H. Inci, U. Topcuoglu, M. Kösoğlu

{"title":"不同摄食策略对蜜蜂肠道菌群及小孢子虫存在的影响","authors":"E. Topal, Ö. Ceylan, R. I. Tunca, V. Bay, S. Aldemir, H. Inci, U. Topcuoglu, M. Kösoğlu","doi":"10.4314/sajas.v52i5.02","DOIUrl":null,"url":null,"abstract":"Colony development in honey bees depends on both the environmental conditions and their genetic structure. Nutrition is the one of the most important factors in the honey bee’s health. This study was carried out in 48 colonies and included six groups (a control and five feeding groups). In the experiment, the effect of different feeding strategies on intestinal flora and the presence of Nosema was investigated. Pantoea agglomerans, Pseudomonas luteola, Burkholderia cepacia, Brevibacillus nitrificans, Sphingomonas paucimobilis, Aeromonas hydrophila, and P. alcalidimonas were detected in the intestinal microflora of the bee samples by morphological and phenotypic identification. The results of phenotypic identification were confirmed using 16S rRNA sequence analysis for P. agglomerans and B. nitrificans strains. The presence of Nosema was simultaneously investigated in all groups. Only Nosema cerenae was detected using DNA analysis in the positive Nosema spore samples.","PeriodicalId":21869,"journal":{"name":"South African Journal of Animal Science","volume":"191 4 1","pages":""},"PeriodicalIF":0.7000,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"The effect of different feeding strategies on honey bee gut microbiota and the presence of Nosema\",\"authors\":\"E. Topal, Ö. Ceylan, R. I. Tunca, V. Bay, S. Aldemir, H. Inci, U. Topcuoglu, M. Kösoğlu\",\"doi\":\"10.4314/sajas.v52i5.02\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Colony development in honey bees depends on both the environmental conditions and their genetic structure. Nutrition is the one of the most important factors in the honey bee’s health. This study was carried out in 48 colonies and included six groups (a control and five feeding groups). In the experiment, the effect of different feeding strategies on intestinal flora and the presence of Nosema was investigated. Pantoea agglomerans, Pseudomonas luteola, Burkholderia cepacia, Brevibacillus nitrificans, Sphingomonas paucimobilis, Aeromonas hydrophila, and P. alcalidimonas were detected in the intestinal microflora of the bee samples by morphological and phenotypic identification. The results of phenotypic identification were confirmed using 16S rRNA sequence analysis for P. agglomerans and B. nitrificans strains. The presence of Nosema was simultaneously investigated in all groups. Only Nosema cerenae was detected using DNA analysis in the positive Nosema spore samples.\",\"PeriodicalId\":21869,\"journal\":{\"name\":\"South African Journal of Animal Science\",\"volume\":\"191 4 1\",\"pages\":\"\"},\"PeriodicalIF\":0.7000,\"publicationDate\":\"2023-04-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"South African Journal of Animal Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.4314/sajas.v52i5.02\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"South African Journal of Animal Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.4314/sajas.v52i5.02","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}

引用次数: 1

摘要

蜜蜂群体的发育取决于环境条件和它们的遗传结构。营养是蜜蜂健康最重要的因素之一。本研究在48个菌落中进行，分为6个组(1个对照组和5个饲养组)。本试验旨在研究不同饲养策略对肉鸡肠道菌群及微孢子虫存在的影响。通过形态学和表型鉴定，在蜜蜂的肠道菌群中检出聚集泛菌、木黄假单胞菌、洋葱伯克氏菌、硝化短芽孢杆菌、少动鞘单胞菌、嗜水气单胞菌和碱性单胞菌。采用16S rRNA序列分析，对团聚假单胞菌和硝化假单胞菌进行表型鉴定。在所有组中同时调查Nosema的存在。阳性小孢子虫样本DNA分析仅检出小孢子虫。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

The effect of different feeding strategies on honey bee gut microbiota and the presence of Nosema

Colony development in honey bees depends on both the environmental conditions and their genetic structure. Nutrition is the one of the most important factors in the honey bee’s health. This study was carried out in 48 colonies and included six groups (a control and five feeding groups). In the experiment, the effect of different feeding strategies on intestinal flora and the presence of Nosema was investigated. Pantoea agglomerans, Pseudomonas luteola, Burkholderia cepacia, Brevibacillus nitrificans, Sphingomonas paucimobilis, Aeromonas hydrophila, and P. alcalidimonas were detected in the intestinal microflora of the bee samples by morphological and phenotypic identification. The results of phenotypic identification were confirmed using 16S rRNA sequence analysis for P. agglomerans and B. nitrificans strains. The presence of Nosema was simultaneously investigated in all groups. Only Nosema cerenae was detected using DNA analysis in the positive Nosema spore samples.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

South African Journal of Animal Science 农林科学-奶制品与动物科学

CiteScore

1.50

自引率

0.00%

发文量

审稿时长

>36 weeks

期刊介绍： The South African Journal of Animal Science is an open access, peer-reviewed journal for publication of original scientific articles and reviews in the field of animal science. The journal publishes reports of research dealing with production of farmed animal species (cattle, sheep, goats, pigs, horses, poultry and ostriches), as well as pertinent aspects of research on aquatic and wildlife species. Disciplines covered nutrition, genetics, physiology, and production systems. Systematic research on animal products, behaviour, and welfare are also invited. Rigorous testing of well-specified hypotheses is expected.