大阳草提取物对子宫内膜异位症小鼠NF-KB、MMP-2和MMP-9表达的影响

IF 0.2 Q4 PHARMACOLOGY & PHARMACY
Annisa Febriani, S. Sutrisno, Yahya Irwanto, Irfani Baihaqi, Wayan Arsana Wiyasa, Bambang Rahardjo
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摘要

子宫内膜异位症是影响育龄妇女生殖健康和生殖功能的最常见疾病。子宫内膜异位症的治疗包括手术和药物治疗。大铃兰是一种原产于印度尼西亚的植物,含有生物活性成分,已知具有抗增殖和细胞凋亡作用。因此,本研究旨在探讨大鳞蟾提取物对子宫内膜异位症小鼠模型(小家鼠)基质金属蛋白酶(MMP-2和MMP-9)及NF-KB表达的影响。方法:本研究采用随机后验,对照组设计。包括六组,即阴性对照组(不含大戟果提取物黄酮类化合物的健康小鼠),阳性对照组(不含大戟果提取物黄酮类化合物的子宫内膜异位症模型)和治疗组,即给予四种不同剂量的大戟果提取物黄酮类化合物的组:每天3.75毫克,每天7.5毫克,每天11.25毫克和每天15毫克。免疫组化染色观察NF-KB、MMP-2和MMP-9的表达,并在40倍显微镜下观察。数据采集采用免疫比对技术,辅以ImageJ分析软件。此外,数据分析采用单因素方差分析方法。结果:在本研究中,四种不同剂量的大戟叶提取物治疗组均可降低MMP-2、MMP-9和NF-KB的表达。高剂量组MMP-2、MMP-9和NF-KB表达降低。结论:在子宫内膜异位症小鼠模型中,大鳞瘤果可降低NF-KB、MMP-2和MMP-9的表达。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of Phaleria macrocarpa Extract on NF-KB, MMP-2, and MMP-9 Expression in Endometriosis Mice Model
Introduction: Endometriosis is the most common disease that affects the reproductive health and function of women of reproductive age. Treatment for endometriosis includes surgery and medication. Phaleria macrocarpa is a plant native to Indonesia that contains bioactive fractions known to have antiproliferative and apoptotic effects. Therefore, this study aimed to evaluate the effect of Phaleria macrocarpa extract on matrix metalloproteinases (MMP-2 and MMP-9) and NF-KB expression in the endometriosis mice model (Mus musculus). Methods: This study used a Randomized Post-Test Only with Control Group Design. Involves six groups, namely a negative control group (healthy mice without flavonoids from Phaleria macrocarpa fruit extract), a positive control group (an endometriosis model without being given flavonoids from Phaleria macrocarpa fruit extract), and a treatment group, namely a group given four different doses of flavonoids from Phaleria macrocarpa fruit extract: 3.75 milligrams per day, 7.5 milligrams per day, 11.25 milligrams per day and 15 milligrams per day. expressions of NF-KB, MMP-2, and MMP-9 were seen using Immunohistochemistry staining and observed under a microscope with 40x magnification. The data collection used an immunoratio technique with ImageJ analysis software. Furthermore, data analysis using the one-way ANOVA method. Results: In this study, the treatment group with four different doses of Phaleria macrocarpa extract could reduce the expression of MMP-2, MMP-9, and NF-KB. Groups with higher doses of Phaleria macrocarpa extract showed lessen of MMP-2, MMP-9, and NF-KB expression. Conclusion: In the endometriosis mice model, Phaleria macrocarpa fruit can reduce NF-KB, MMP-2, and MMP-9 expression.
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