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引用次数: 2
摘要
饮用水消毒效率的评估传统上包括以可培养菌数减少的形式估计粪便指示剂的失活率。广泛描述的，作为细菌在少营养条件下生存策略的一种形式的可存活但不可培养(VBNC)状态在这些估计中没有被考虑。本研究的目的是评估游离氯消毒对粪便指示剂大肠杆菌的影响，以研究氯暴露后细胞变化的演替。为了确定失活率，研究了细胞形成菌落的能力、分裂能力等和代谢活性。需要0.064 mg/L min -1游离氯才能使可培养计数失活99%;然而，5 - 200倍的消毒剂剂量x接触时间分别会降低细胞的分裂能力和代谢活性。结果表明，为了确定正确的饮用水消毒剂剂量，需要对多个细胞活力参数进行分析。
The Effect of Chlorination on Escherichia Coli Viability in Drinking Water
The assessment of drinking water disinfection efficiency traditionally involves the estimation of faecal indicator inactivation rates in the form of reduction of cultivable counts. Widely described, viable but noncultivable (VBNC) state as a form of bacterial survival strategy in oligotrophic conditions is not considered in these estimations. The aim of the present research is to assess the effect of free chlorine disinfection on faecal indicator Escherichia coli in order to study the succession of cellular alterations in response to chlorine exposure. To determine the inactivation rates, cell ability to form colonies, ability to divide as such and metabolic activity have studied. 0.064 mg/L min -1 free chlorine has been needed to obtain 99 % inactivation of cultivable counts; however, 5 till 200 times higher disinfectant dose x contact time has been required to reduce cell ability to divide and metabolic activity, respectively. The results have shown that to determine correct drinking water disinfectant doses multiple cell viability parameters should be analyzed.
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