抗生素耐药菌β -内酰胺酶基因比色生物芯片的杂交分析

http://eng.biomos.ru/conference/articles.htm Pub Date : 2021-01-01 DOI:10.37747/2312-640x-2021-19-252-254

М.Y. Rubtsova, N. Fursova, G. Presnova, M. Ulyashova, V. Grigorenko, А. Egorov

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引用次数: 0

摘要

建立了一种利用比色生物芯片进行核酸定量分析的杂交分析方法。优化了用不同颜色模型处理生物芯片数字图像的实验条件和方法。本方法用于耐药革兰氏阴性菌中丝氨酸-内酰胺酶DNA和mRNA的定量测定。

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HYBRIDIZATION ANALYSIS ON COLORIMETRIC BIOCHIPS FOR QUANTITATIVE DETERMINATION OF BETA-LACTAMASE GENES IN ANTIBIOTIC RESISTANT BACTERIA

A method of hybridization analysis on colorimetric biochips for the quantitative determination of nucleic acids has been developed. The assay conditions and the method of processing digital images of biochips using different color models have been optimized. The method was applied for the quantitative determination of DNA and mRNA of serine beta-lactamases in Gram-negative bacteria resistant to beta-lactam antibiotics.

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