Vanesa Vebiola Kumakauw, H. Simbala, K. L. Mansauda
{"title":"葡萄球菌菌和沙门氏菌的抗菌提取物","authors":"Vanesa Vebiola Kumakauw, H. Simbala, K. L. Mansauda","doi":"10.35799/JMUO.9.2.2020.28946","DOIUrl":null,"url":null,"abstract":"Sesewanua (Clerodendron squamatum Vahl.) secara empiris telah digunakan oleh masyarakat dibeberapa daerah di Sulawesi Utara untuk mengobati berbagai macam penyakit. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri dari ekstrak etanol daun sesewanua terhadap daya hambat pertumbuhan bakteri Staphylococcus aureus, Escherichia coli dan Salmonella typhi menggunakan tiga kosentrasi yakni 20%, 40% dan 60%. Ekstraksi dilakukan dengan cara maserasi menggunakan etanol 96%. Pengujian aktivitas antibakteri menggunakan metode difusi dengan cakram kertas (difusi Kirby dan Bauer). Hasil penelitian ini menunjukkan bahwa ekstrak etanol daun sesewanua berpotensi sebagai antibakteri dan memiliki kekuatan antibakteri terhadap Staphylococcus aureus, Escherichia coli dan Salmonella typhi, pada kosentrasi 20%, 40% dan 60% termasuk golongan kuat untuk menghambat bakteri Staphylococcus aureus, sedangkan pada kosentrasi 40% dan 60% termasuk dalam golongan kuat untuk menghambat bakteri Escherichia coli dan Salmonella typhi.Sesewanua (Clerodendron squamatum Vahl.) have been used by communities in North Sulawesi for to treat or handle various diseases. This study aims to determine the antibacterial activity from ethanol extract Sesewanua Leaves against the growth inhibition of the bacterium Staphylococcus aureus, Escherichia coli and Salmonella typhi using three concentrations of 20%, 40% and 60%. Extraction was done by maceration method using ethanol 96%. Antibacterial activity test was performing using the paper disk diffusion method (Kirby and Bauer diffusion). This results of this study indicate that ethanol extracts of Sesewanua leaves are considered as antibacterial and have antibacterial strength against Staphylococcus aureus, Escherichia coli and Salmonella typhi, at concentrations of 20%, 40% and 60% include strong groups to inhibit Staphylococcus aureus, while at a concentration of 40% and 60% include strong groups to inhibit Escherichia coli and Salmonella typhi.","PeriodicalId":53333,"journal":{"name":"Jurnal MIPA","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2020-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"6","resultStr":"{\"title\":\"Aktivitas Antibakteri Ekstrak Etanol Daun Sesewanua (Clerodendron Squamatum Vahl.) terhadap Bakteri Staphylococcus aureus Escherichia coli dan Salmonella typhi\",\"authors\":\"Vanesa Vebiola Kumakauw, H. Simbala, K. L. 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Hasil penelitian ini menunjukkan bahwa ekstrak etanol daun sesewanua berpotensi sebagai antibakteri dan memiliki kekuatan antibakteri terhadap Staphylococcus aureus, Escherichia coli dan Salmonella typhi, pada kosentrasi 20%, 40% dan 60% termasuk golongan kuat untuk menghambat bakteri Staphylococcus aureus, sedangkan pada kosentrasi 40% dan 60% termasuk dalam golongan kuat untuk menghambat bakteri Escherichia coli dan Salmonella typhi.Sesewanua (Clerodendron squamatum Vahl.) have been used by communities in North Sulawesi for to treat or handle various diseases. This study aims to determine the antibacterial activity from ethanol extract Sesewanua Leaves against the growth inhibition of the bacterium Staphylococcus aureus, Escherichia coli and Salmonella typhi using three concentrations of 20%, 40% and 60%. Extraction was done by maceration method using ethanol 96%. Antibacterial activity test was performing using the paper disk diffusion method (Kirby and Bauer diffusion). 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引用次数: 6
摘要
Sesewanua (Clerodendron squamatum Vahl)曾被北苏拉威西几个地区的社区用过治疗各种疾病。该研究的目的是确定从葡萄球菌菌根、Escherichia大肠杆菌和沙门氏杆菌中提取出的抗菌活性，该菌以20%、40%和60%为中心。提取方法是用96%的乙醇进行的。利用柯比和鲍尔的文件扩散方法测试抗菌活性。这项研究结果表明,潜在sesewanua叶乙醇提取物作为杆菌抗菌抗菌和有能力对奥里斯,Escherichia大肠杆菌和沙门氏菌typhi在关注20%、40%和60%,包括阶级强大可以抑制细菌体内奥里斯,而在阶级强大关注40%和60%包括抑制细菌Escherichia大肠杆菌和沙门氏菌typhi。Sesewanua (clerodentum squamatum Vahl)曾被北苏拉威西社区用来治疗各种疾病。这项研究旨在确定来自乙醇extract sewanua的抗菌活性抑制，导致大肠杆菌、大肠杆菌和沙门氏菌的生长抑制，使用20%、40%和60%的三分流酶。用乙醇96%的方法进行的提取。防细菌活动测试是在纸盘离散方式上进行的。这个results of This study indicate that Sesewanua树叶是认为美国的乙醇摘录antibacterial和体内有antibacterial力量反对奥里斯,Escherichia大肠杆菌和沙门氏菌typhi, at concentrations of 20%、40%和60% include特朗集团杆菌inhibit奥里斯,而在百万双臀40%和60%的include特朗集团到inhibit Escherichia大肠杆菌和沙门氏菌typhi。
Aktivitas Antibakteri Ekstrak Etanol Daun Sesewanua (Clerodendron Squamatum Vahl.) terhadap Bakteri Staphylococcus aureus Escherichia coli dan Salmonella typhi
Sesewanua (Clerodendron squamatum Vahl.) secara empiris telah digunakan oleh masyarakat dibeberapa daerah di Sulawesi Utara untuk mengobati berbagai macam penyakit. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri dari ekstrak etanol daun sesewanua terhadap daya hambat pertumbuhan bakteri Staphylococcus aureus, Escherichia coli dan Salmonella typhi menggunakan tiga kosentrasi yakni 20%, 40% dan 60%. Ekstraksi dilakukan dengan cara maserasi menggunakan etanol 96%. Pengujian aktivitas antibakteri menggunakan metode difusi dengan cakram kertas (difusi Kirby dan Bauer). Hasil penelitian ini menunjukkan bahwa ekstrak etanol daun sesewanua berpotensi sebagai antibakteri dan memiliki kekuatan antibakteri terhadap Staphylococcus aureus, Escherichia coli dan Salmonella typhi, pada kosentrasi 20%, 40% dan 60% termasuk golongan kuat untuk menghambat bakteri Staphylococcus aureus, sedangkan pada kosentrasi 40% dan 60% termasuk dalam golongan kuat untuk menghambat bakteri Escherichia coli dan Salmonella typhi.Sesewanua (Clerodendron squamatum Vahl.) have been used by communities in North Sulawesi for to treat or handle various diseases. This study aims to determine the antibacterial activity from ethanol extract Sesewanua Leaves against the growth inhibition of the bacterium Staphylococcus aureus, Escherichia coli and Salmonella typhi using three concentrations of 20%, 40% and 60%. Extraction was done by maceration method using ethanol 96%. Antibacterial activity test was performing using the paper disk diffusion method (Kirby and Bauer diffusion). This results of this study indicate that ethanol extracts of Sesewanua leaves are considered as antibacterial and have antibacterial strength against Staphylococcus aureus, Escherichia coli and Salmonella typhi, at concentrations of 20%, 40% and 60% include strong groups to inhibit Staphylococcus aureus, while at a concentration of 40% and 60% include strong groups to inhibit Escherichia coli and Salmonella typhi.
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