用计算机辅助精子分析仪评价雄鹿不同遗传群的精子活力和速度

M. Islam, M. Talukder, M. Rahman, S. S. Husain
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引用次数: 4

摘要

精液质量评估是任何育种计划的首要先决条件。计算机辅助精子分析仪(CASA)能够准确、灵敏地测定精液质量。该研究是在加济浦尔斯里普尔万纳哈蒂的美国乳业有限公司(ADL)进行的。从黑孟加拉(BB)、布尔(Boer)和贾穆纳帕利(Jamunapari)三个遗传群体中收集了120次射精,目的是利用计算机辅助精子分析仪来描述和比较精液活力和速度参数。差异均显著(p0.05)。所有基因型的阴囊周长与精液量和曲线速度均呈正相关。结果表明,布尔雄鹿的精液质量始终优于其他两个遗传群体。此外,可以进行大样本的竞争性研究,以评估现有山羊品种的品种特异性差异。我认为本研究还揭示了计算机辅助精子分析仪的精液质量的一些罕见的参数,这是人工无法做到的。本研究结果可为今后不同遗传群体雄鹿的性能测试提供可靠的指导。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of sperm motility and velocity of different genetic groups of bucks using Computer-Assisted Sperm Analyzer
Semen quality assessment is a prime prerequisite for any breeding programme. Computer-Assisted Sperm Analyzer (CASA) can measure the semen quality very accurately and sharply. The research was conducted at American Dairy Limited (ADL) at Vangnahati, Sreepur, Gazipur. A total of 120 ejaculates were collected from three genetic groups of bucks namely Black Bengal (BB), Boer and Jamunapari (JP) with a view to characterize and compare the semen motility and velocity parameters using ComputerAssisted Sperm Analyser. Significantly (p<0.05) higher ejaculate volume was found in Boer buck (1.73±0.16 ml) with the lower in BB buck (0.98±0.14), respectively. But significantly (p<0.05) higher total, forward progressive and fast motility was found in BB (55.47±3.17, 47.23±2.74 and 37.62±2.40%) and lower in JP (40.67±3.37, 30.97±2.91 and 21.81±2.55%), respectively. Significantly (p<0.01) fastest straight line and average path distance was observed in Boer bucks (12.29±1.63 and 15.89±1.95 μm) than Black Bengal and Jamunapari bucks (4.38±1.19 and 7.26±1.57; and 3.14±1.27 and 8.42±1.95 μm), respectively. The distance curved line (DCL) and distance straight line (DSL) was significantly (p<0.05) higher in Boer (29.13±4.87, 12.29±1.63 and 15.89±1.95 m/sec) and lower in BB (22.07±4.36 and 7.26±1.57 m/sec), respectively. In case of curvilinear velocity, straight line velocity and average path velocity value was significantly (p<0.01) higher in Boer (127.08±13.88, 50.01±7.68 and 68.13±7.25 m/sec) with lower in JP (62.01±12.77, 15.75±7.35 and 22.39±9.42) where as linearity and straightness was higher in Boer and lower in BB (p>0.05), respectively. The scrotal circumference had positive correlations with semen volume and curvilinear velocity for all the genotypes. It was concluded that semen of Boer bucks found consistently better than other two genetic groups of bucks. Further, competitive studies with large sample may be performed to evaluate the breed specific differences in available goat breeds. I think this study also disclosed the some rare parameters of semen quality by using Computer-Assisted Sperm Analyzer which was impossible in manually. These results of the present study could be an authentic guideline in future performance tests of different genetic groups of bucks.
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